荷兰一家大型三级中心慢性乙型肝炎病毒携带者中丁型肝炎病毒的流行情况。

Prevalence of hepatitis delta virus among chronic hepatitis B carriers in a large tertiary center in the Netherlands.

机构信息

Erasmus MC University Medical Center, Department of Viroscience, Rotterdam, the Netherlands; Erasmus MC University Medical Center, Department of Gastroenterology and Hepatology, Rotterdam, the Netherlands.

Erasmus MC University Medical Center, Department of Viroscience, Rotterdam, the Netherlands.

出版信息

J Clin Virol. 2021 Aug;141:104870. doi: 10.1016/j.jcv.2021.104870. Epub 2021 Jun 18.

DOI:10.1016/j.jcv.2021.104870

PMID:34182298

Abstract

BACKGROUND & AIMS: Hepatitis D virus infection (HDV) is considered the most severe form of viral hepatitis. In this study, we aimed to evaluate the prevalence of HDV infection in a tertiary center of a large, multi-ethnic city in the Netherlands. Moreover, we validate the reliability of a novel anti-HDV CLIA assay.

METHODS

All HBsAg-positive patients visiting the outpatient clinic between 2017 and 2019 were tested for HDV serology. Seropositive serum samples were further assessed by HDV RNA PCR and Sanger sequencing to identify the HDV genotype.

RESULTS

The CLIA assay was 100% sensitive and 98% specific. Out of 925 patients 3.7% tested seropositive for HDV, and HDV viremia was confirmed in 2.0%. The majority of patients had a non-Dutch background and did not speak English or Dutch. We detected HDV genotype 5 (N = 3), and genotype 1 (N = 15). Phylogenetic analysis demonstrated HDV1 clusters composed of sub-Saharan Africa isolates, central Asian, Turkish, Iranian and European isolates.

CONCLUSIONS

The prevalence of HDV infection in a tertiary center in the Netherlands was 2.0% among HBsAg-positive individuals, and mainly in non-Dutch individuals. Only HDV genotype 1 and 5 isolates were detected, which was found to match with the patient's country of origin.

摘要

背景与目的

丁型肝炎病毒（HDV）感染被认为是最严重的病毒性肝炎形式。本研究旨在评估荷兰一个多民族大城市的三级中心的 HDV 感染流行率。此外，我们还验证了一种新型抗-HDV CLIA 检测的可靠性。

方法

在 2017 年至 2019 年间，所有 HBsAg 阳性就诊于门诊的患者均接受了 HDV 血清学检测。对血清阳性样本进一步进行 HDV RNA PCR 和 Sanger 测序，以确定 HDV 基因型。

结果

CLIA 检测的敏感性为 100%，特异性为 98%。在 925 例患者中，有 3.7%的患者 HDV 血清学检测呈阳性，且有 2.0%的患者证实存在 HDV 病毒血症。大多数患者来自非荷兰背景，且不会说英语或荷兰语。我们检测到 HDV 基因型 5（N=3）和基因型 1（N=15）。系统进化分析显示，HDV1 聚类由撒哈拉以南非洲、中亚、土耳其、伊朗和欧洲的分离株组成。