Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China; Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province, Wanning, Hainan 571533, China.
Department of Pharmacology, School of Basic Medicine and Life Science, Hainan Medical University, Haikou, Hainan 571199, China.
Food Chem. 2021 Dec 1;364:130434. doi: 10.1016/j.foodchem.2021.130434. Epub 2021 Jun 23.
This study aimed to investigate the effects of polysaccharide from Artocarpus heterophyllus Lam. pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice. The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods. The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID). The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7. The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks. These results indicate that JFP-Ps is beneficial to the gut health and can be developed as a functional ingredient in relation to gut health.
本研究旨在探讨荔枝果肉多糖（JFP-Ps）对小鼠肠道微生物组成和短链脂肪酸产生的影响。采用 PCR 扩增 V3 和 V4 区 16S rRNA 基因，然后在 Illumina MiSeq PE250 平台上进行测序，并采用多变量统计方法进行分析。采用配备火焰离子化检测器（FID）的气相色谱（GC）法测定短链脂肪酸（SCFAs）的浓度。结果表明，JFP-Ps 显著影响肠道细菌的水平，包括拟杆菌门、厚壁菌门、变形菌门、蓝藻门、放线菌门、无壁菌门、脱铁杆菌门和 TM7。用 JFP-Ps 处理 2 周后，小鼠粪便中乙酸、丙酸、正丁酸和总 SCFAs 的浓度显著增加。这些结果表明，JFP-Ps 有益于肠道健康，可以开发为与肠道健康相关的功能性成分。
