Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, China.
School of Food Science and Engineering, Hainan University, Haikou 570228, China.
Nutrients. 2024 Jan 4;16(1):166. doi: 10.3390/nu16010166.
In recent years, Lam. (jackfruit) polysaccharides (namely JFP-Ps) have attracted much attention due to their multiple biological activities. This study aimed to explore the protective effects and the underlying mechanisms of JFP-Ps on cyclophosphamide (Cp)-induced liver damage. The protective effect of JFP-Ps was evaluated using HE staining, antioxidant testing, enzyme-linked immunosorbent assay (ELISA), real-time quantitative polymerase chain reaction (RT-qPCR), Western blot and ultra-performance liquid chromatography equipped with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) metabolomics analysis. The results showed that Cp caused pathological liver damage, activated oxidative stress and downregulated cytokine expression, while JFP-Ps treatment was found to exert antioxidant effects and play immune regulatory roles through mitogen-activated protein kinase/nuclear factor-κB (MAPK/NF-κB) related inflammation and cell apoptosis pathways to protect the Cp-induced liver injury. Metabolomic results showed that the liver-protective effects of JFP-Ps were mainly related to aminoacyl transfer ribonucleic acid (tRNA) biosynthesis, sphingolipid metabolism, purine metabolism and the citrate cycle. These results indicate that JFP-Ps have great potential application in alleviating liver injury.
近年来，Lam.（菠萝蜜）多糖（即 JFP-Ps）因其多种生物活性而备受关注。本研究旨在探讨 JFP-Ps 对环磷酰胺（Cp）诱导的肝损伤的保护作用及其机制。采用 HE 染色、抗氧化试验、酶联免疫吸附试验（ELISA）、实时定量聚合酶链反应（RT-qPCR）、Western blot 和超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF-MS/MS）代谢组学分析评估 JFP-Ps 的保护作用。结果表明，Cp 可导致肝组织病理学损伤，激活氧化应激，下调细胞因子表达，而 JFP-Ps 处理可通过丝裂原活化蛋白激酶/核因子-κB（MAPK/NF-κB）相关炎症和细胞凋亡途径发挥抗氧化作用和免疫调节作用，从而保护 Cp 诱导的肝损伤。代谢组学结果表明，JFP-Ps 的肝保护作用主要与氨酰转移 RNA（tRNA）生物合成、鞘脂代谢、嘌呤代谢和柠檬酸循环有关。这些结果表明，JFP-Ps 在缓解肝损伤方面具有很大的应用潜力。
