Zeng Shunjiang, Cao Jun, Chen Yuzi, Li Chuan, Wu Gang, Zhu Kexue, Chen Xiaoai, Xu Fei, Liu Qibing, Tan Lehe
Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, China.
College of Food Science and Engineering, Hainan University, Haikou, China.
Front Nutr. 2022 Nov 3;9:1035619. doi: 10.3389/fnut.2022.1035619. eCollection 2022.
Polysaccharides show protective effects on intestinal barrier function due to their effectiveness in mitigating oxidative damage, inflammation and probiotic effects. Little has been known about the effects of polysaccharides from Lam. pulp (jackfruit, JFP-Ps) on intestinal barrier function. This study aimed to investigate the effects of JFP-Ps on intestinal barrier function in high fat diet-induced obese rats. H&E staining and biochemical analysis were performed to measure the pathological and inflammatory state of the intestine as well as oxidative damage. Expression of the genes and proteins associated with intestinal health and inflammation were analyzed by RT-qPCR and western blots. Results showed that JFP-Ps promoted bowel movements and modified intestinal physiochemical environment by lowering fecal pH and increasing fecal water content. JFP-Ps also alleviated oxidative damage of the colon, relieved intestinal colonic inflammation, and regulated blood glucose transport in the small intestine. In addition, JFP-Ps modified intestinal physiological status through repairing intestinal mucosal damage and increasing the thickness of the mucus layer. Furthermore, JFP-Ps downregulated the inflammatory genes (TNF-α, IL-6) and up-regulated the free fatty acid receptors (GPR41 and GPR43) and tight junction protein (occludin). These results revealed that JFP-Ps showed a protective effect on intestinal function through enhancing the biological, mucosal, immune and mechanical barrier functions of the intestine, and activating SCFAs-GPR41/GPR43 related signaling pathways. JFP-Ps may be used as a promising phytochemical to improve human intestinal health.
由于多糖在减轻氧化损伤、炎症和益生菌作用方面的有效性，其对肠道屏障功能具有保护作用。关于波罗蜜果肉多糖（菠萝蜜多糖，JFP-Ps）对肠道屏障功能的影响，人们了解甚少。本研究旨在探讨JFP-Ps对高脂饮食诱导的肥胖大鼠肠道屏障功能的影响。通过苏木精-伊红（H&E）染色和生化分析来测量肠道的病理和炎症状态以及氧化损伤。通过逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹法分析与肠道健康和炎症相关的基因和蛋白质的表达。结果表明，JFP-Ps促进肠道蠕动，并通过降低粪便pH值和增加粪便含水量来改善肠道理化环境。JFP-Ps还减轻了结肠的氧化损伤，缓解了肠道炎症，并调节了小肠中的血糖转运。此外，JFP-Ps通过修复肠道黏膜损伤和增加黏液层厚度来改善肠道生理状态。此外，JFP-Ps下调炎症基因（肿瘤坏死因子-α、白细胞介素-6），上调游离脂肪酸受体（GPR41和GPR43）和紧密连接蛋白（闭合蛋白）。这些结果表明，JFP-Ps通过增强肠道的生物、黏膜、免疫和机械屏障功能以及激活短链脂肪酸-GPR41/GPR43相关信号通路，对肠道功能具有保护作用。JFP-Ps可能作为一种有前景的植物化学物质来改善人类肠道健康。
