促进非小细胞肺癌和头颈部鳞状细胞癌细胞的增殖。
Promotes Proliferation of Non-small-cell Lung Cancer and Head and Neck Squamous Cell Carcinoma Cells.
机构信息
Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan;
Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan.
出版信息
Cancer Genomics Proteomics. 2021 Jul-Aug;18(4):543-548. doi: 10.21873/cgp.20279.
BACKGROUND/AIM: The long noncoding RNA OIP5 antisense RNA 1 (OIP5-AS1) is overexpressed in various cancer types, such as lung cancer, hepatoblastoma and cervical cancer, and functions to accelerate cell proliferation, invasion and migration. Here, we investigated the roIe of OIP5-AS1 in cell-cycle progression of H1299 and A549 non-small cell lung cancer cells, and FaDu and CAL27 head and neck squamous cell carcinoma cells.
MATERIALS AND METHODS
The cells were transfected with small interfering RNA and subjected to cell-cycle analysis and reverse-transcription quantitative polymerase chain reaction (RT-qPCR).
RESULTS
Silencing of OIP5-AS1 suppressed the proliferation of H1299, A549, FaDu and CAL27 cells. RT-qPCR and cell-cycle analysis revealed that silencing OIP5-AS1 increased the expression of CDK inhibitors, such as p15, p16, p18 and p19, resulting in G1-phase arrest.
CONCLUSION
OIP5-AS1 regulates G1-phase progression by repressing CDK inhibitors and, thus, promotes the proliferation of H1299, A549, FaDu and CAL27 cells.
背景/目的：长链非编码 RNA OIP5 反义 RNA 1（OIP5-AS1）在多种癌症类型中过表达，如肺癌、肝母细胞瘤和宫颈癌，其功能是加速细胞增殖、侵袭和迁移。在这里，我们研究了 OIP5-AS1 在 H1299 和 A549 非小细胞肺癌细胞以及 FaDu 和 CAL27 头颈部鳞状细胞癌细胞细胞周期进展中的作用。
材料和方法
用小干扰 RNA 转染细胞，并进行细胞周期分析和逆转录定量聚合酶链反应（RT-qPCR）。
结果
沉默 OIP5-AS1 抑制了 H1299、A549、FaDu 和 CAL27 细胞的增殖。RT-qPCR 和细胞周期分析显示，沉默 OIP5-AS1 增加了 CDK 抑制剂的表达，如 p15、p16、p18 和 p19，导致 G1 期阻滞。
结论
OIP5-AS1 通过抑制 CDK 抑制剂来调节 G1 期进展，从而促进 H1299、A549、FaDu 和 CAL27 细胞的增殖。
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