Fattah Abolfazl, Morovati Ali, Niknam Zahra, Mashouri Ladan, Asadi Amirhooman, Rizi Shirin Tvangar, Abbasi Mojtaba, Shakeri Fatemeh, Abazari Omid
Research Center for Health Sciences and Technologies, Semnan University of Medical Sciences, Semnan, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Iran J Public Health. 2021 May;50(5):1037-1047. doi: 10.18502/ijph.v50i5.6121.
Piperine is a natural compound obtained from the that exhibits anti-proliferative and anti-cancer activity in cancer cell lines. We analyzed the cytotoxic effect of piperine combined with cisplatin compound in the human MCF-7 breast cancer cell line and the underlying mechanism.
The present in vitro study was performed on MCF-7 cell line in Jahrom University of Medical Sciences between, Jahrom, Iran from 2016 to 2017. Cultured MCF-7 cells were seeded into four groups: a control group (untreated group), a group treated with cisplatin, a group treated with piperine and a group treated with cisplatin and piperine. Cell viability was analyzed using the MTT assay method. Flow c-ytometric analysis was investigated for apoptosis. The mRNA and protein expression of the apoptotic regulators p53, Bcl-2, Bax, caspase 3 and caspase 9 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis.
Piperine (20 and 30 μM) in combination with cisplatin (5, 10 and 15 μM) for 24 h synergistically inhibited cell viability of MCF-7 breast cancer cells more than piperine and cisplatin used alone. Synergistic anti-breast cancer activities cisplatin (5 μM) and piperine (20 μM) were via inducing apoptosis. Piperine (20 μM) and cisplatin (5 μM) for 24 h induce apoptosis strongly through reduction of Bcl-2 and increase of caspase 3, p53, caspase 9, and Bax.
Piperine in combination with cisplatin could trigger p53-mediated apoptosis more effective than cisplatin alone in MCF-7 breast cancer cells, reducing the toxic dose of cisplatin used in cancer chemotherapy.
胡椒碱是一种从[此处原文缺失相关内容]中提取的天然化合物，在癌细胞系中具有抗增殖和抗癌活性。我们分析了胡椒碱与顺铂联合用药对人MCF - 7乳腺癌细胞系的细胞毒性作用及其潜在机制。
本体外研究于2016年至2017年在伊朗贾赫罗姆医科大学对MCF - 7细胞系进行。将培养的MCF - 7细胞接种到四组中：对照组（未处理组）、顺铂处理组、胡椒碱处理组和顺铂与胡椒碱联合处理组。使用MTT分析法分析细胞活力。通过流式细胞术分析凋亡情况。通过定量实时聚合酶链反应（qRT - PCR）和蛋白质印迹分析检测凋亡调节因子p53、Bcl - 2、Bax、半胱天冬酶3和半胱天冬酶9的mRNA和蛋白质表达。
胡椒碱（20和30μM）与顺铂（5、10和15μM）联合作用24小时，比单独使用胡椒碱和顺铂更能协同抑制MCF - 7乳腺癌细胞的活力。顺铂（5μM）和胡椒碱（20μM）的协同抗乳腺癌活性是通过诱导凋亡实现的。胡椒碱（20μM）和顺铂（5μM）作用24小时通过降低Bcl - 2并增加半胱天冬酶3、p53、半胱天冬酶9和Bax来强烈诱导凋亡。
在MCF - 7乳腺癌细胞中，胡椒碱与顺铂联合用药比单独使用顺铂更能有效地触发p53介导的凋亡，从而降低癌症化疗中顺铂的使用剂量。
