Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Department of Ophthalmology, Aarhus University Hospital, Aarhus, Denmark.
Invest Ophthalmol Vis Sci. 2022 Aug 2;63(9):11. doi: 10.1167/iovs.63.9.11.
Animal models of choroidal neovascularization (CNV) are extensively used to characterize the pathophysiology of chorioretinal diseases with CNV formation and to evaluate novel treatment strategies. This systematic review aims to give a detailed overview of contemporary animal models of CNV.
A systematic search was performed in PubMed and EMBASE from November 20, 2015, to November 20, 2020, for mammalian animal models of CNV. Following inclusion by two investigators, data from the articles were extracted according to a predefined protocol.
A total of 380 full articles, representing 409 independent animal models, were included. Mice were by far the most utilized animal (76%) followed by rats and non-human primates. The median age of rodents was 8 weeks but with a wide range. Male animals were used in 44% of the studies, but 32% did not report the sex. CNV was laser induced in 89% of the studies, but only 44% of these reported sufficiently on standard laser parameters. Surprisingly, 28% of the studies did not report a sample size for quantitative CNV evaluation. Less than half of the studies performed quantitative in vivo evaluation, and 73% evaluated CNV quantitatively ex vivo. Both in vivo and ex vivo evaluations were conducted primarily at day 7 and/or day 14.
The laser-induced mouse model is the predominant model for experimental CNV. The widespread use of young, healthy male animals may complicate clinical translation, and inadequate reporting challenges reproducibility. Definition and implementation of standardized methodologic and reporting guidelines are attractive.
脉络膜新生血管（CNV）动物模型被广泛用于研究伴有 CNV 形成的眼后段疾病的病理生理学，并评估新的治疗策略。本系统综述旨在详细概述当前的 CNV 动物模型。
我们于 2015 年 11 月 20 日至 2020 年 11 月 20 日在 PubMed 和 EMBASE 中进行了系统性检索，以获取 CNV 哺乳动物动物模型的相关研究。两名研究者进行纳入后，根据预先设定的方案提取文章中的数据。
共纳入 380 篇全文，代表 409 个独立的动物模型。小鼠是迄今为止使用最广泛的动物（76%），其次是大鼠和非人类灵长类动物。啮齿动物的中位年龄为 8 周，但范围较宽。44%的研究使用雄性动物，但 32%的研究未报告性别。89%的研究采用激光诱导 CNV，但仅有 44%的研究充分报告了标准激光参数。令人惊讶的是，28%的研究未报告定量 CNV 评估的样本量。不到一半的研究进行了定量体内评估，73%的研究进行了定量离体评估。体内和离体评估主要在第 7 天和/或第 14 天进行。
激光诱导的小鼠模型是实验性 CNV 的主要模型。广泛使用年轻、健康的雄性动物可能会使临床转化复杂化，并且不充分的报告也会对可重复性造成挑战。制定和实施标准化的方法学和报告指南具有吸引力。
