Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas. Ciudad de México, México.
Ecology of Pathogen Agents Department, General Hospital Manuel Gea González, Mexico City, Mexico.
PLoS Negl Trop Dis. 2021 Jun 29;15(6):e0009541. doi: 10.1371/journal.pntd.0009541. eCollection 2021 Jun.
Burkholderia sensu stricto is comprised mainly of opportunistic pathogens. This group is widely distributed in the environment but is especially important in clinical settings. In Mexico, few species have been correctly identified among patients, most often B. cepacia is described.
METHODOLOGY/PRINCIPAL FINDINGS: In this study, approximately 90 strains identified as B. cepacia with the VITEK2 system were isolated from two medical centers in Mexico City and analyzed by MLSA, BOX-PCR and genome analysis. The initial identification of B. cepacia was confirmed for many strains, but B. contaminans, B. multivorans and B. vietnamiensis were also identified among clinical strains for the first time in hospitals in Mexico. Additionally, the presence of B. pseudomallei was confirmed, and a novel species within the B. cepacia complex was documented. Several strains misidentified as B. cepacia actually belong to the genera Pseudomonas, Stenotrophomonas and Providencia.
CONCLUSIONS/SIGNIFICANCE: The presence of different Burkholderia species in Mexico was confirmed. Correct identification of Burkholderia species is important to provide accurate treatment for immunosuppressed patients.
严格意义上的伯克霍尔德菌主要由机会性病原体组成。该群体广泛分布于环境中，但在临床环境中尤为重要。在墨西哥，很少有患者的正确鉴定出的物种，通常描述为洋葱伯克霍尔德菌。
方法/主要发现：在这项研究中，大约 90 株经 VITEK2 系统鉴定为洋葱伯克霍尔德菌的菌株从墨西哥城的两个医疗中心分离出来，并通过 MLSA、BOX-PCR 和基因组分析进行分析。许多菌株的初始鉴定为洋葱伯克霍尔德菌，但首次在墨西哥医院的临床菌株中鉴定出了污染伯克霍尔德菌、多维多糖伯克霍尔德菌和越南伯克霍尔德菌。此外，还证实了类鼻疽伯克霍尔德菌的存在，并记录了洋葱伯克霍尔德菌复合体中的一个新种。一些被错误鉴定为洋葱伯克霍尔德菌的菌株实际上属于假单胞菌属、寡养单胞菌属和普罗维登斯菌属。
结论/意义：证实了不同的伯克霍尔德菌物种在墨西哥的存在。正确鉴定伯克霍尔德菌物种对于为免疫抑制患者提供准确治疗非常重要。
