PG and Research Centre in Biotechnology, MGR College, Adhiyamaan Educational Research Institute, Hosur, Tamil Nadu, India.
Natural Drug Research Laboratory, Department of Biotechnology, Periyar University, Salem, Tamil Nadu, India.
Environ Res. 2021 Oct;201:111594. doi: 10.1016/j.envres.2021.111594. Epub 2021 Jun 26.
This research was aimed to evaluate the phytochemical profile, bactericidal activity of Hygrophila spinosa against multidrug resistant Pandoraea sputorum and assess their antioxidant competence against various radicals and studied their hepatoprotective and nephroprotective activity on HepG2 and HEK 293 cell line. The results showed that the methanol extract has various phytochemical components with reasonable quantity. Fortunately, the multidrug-resistant P. sputorum was sensitive (22.8 ± 0.2 mm of the zone of inhibition) at 15 mg mL concentration of methanol extract. The higher concentration of phenolic and other phytochemical components, showed significant antioxidant activity against ferric, DPPH, hydroxyl, and ABTS radicals, with IC values of 71.09, 64.333, 91.157, and 104.931 g mL, respectively. Surprisingly, the methanol extract possesses hepato and nephroprotective activity against CCl and cisplatin-induced cytotoxicity on HepG2 and HEK 293 cell lines, respectively. It maintains the cell viability as up to 90.48% and 90.35% of HepG2 and EK 293 cell line at the concentration of 20 μg mL. The FTIR analysis states that the methanol extract possesses a significant functional group responsible for these multi-potential activities. These results suggest that, the methanol extract of H. spinosa might contain the most significant bioactive components with outstanding medicinal properties.
这项研究旨在评估 Hygrophila spinosa 的植物化学成分谱、对多药耐药 Pandoraea sputorum 的杀菌活性，并评估其对各种自由基的抗氧化能力，并研究其对 HepG2 和 HEK 293 细胞系的肝保护和肾保护活性。结果表明，甲醇提取物具有各种具有合理数量的植物化学成分。幸运的是，多药耐药的 P. sputorum 对 15mg mL 浓度的甲醇提取物敏感（抑制区为 22.8±0.2mm）。酚类和其他植物化学成分的浓度越高，对铁、DPPH、羟基和 ABTS 自由基的抗氧化活性就越显著，IC 值分别为 71.09、64.333、91.157 和 104.931g mL。令人惊讶的是，甲醇提取物对 HepG2 和 HEK 293 细胞系中的 CCl 和顺铂诱导的细胞毒性具有肝保护和肾保护活性，在 20μg mL 的浓度下，分别保持高达 90.48%和 90.35%的细胞活力。FTIR 分析表明，甲醇提取物具有负责这些多效性活动的重要功能基团。这些结果表明，H. spinosa 的甲醇提取物可能含有具有突出药用特性的最重要的生物活性成分。
