Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Bioresour Technol. 2021 Oct;337:125447. doi: 10.1016/j.biortech.2021.125447. Epub 2021 Jun 24.
The microalga Poterioochromonas malhamensis was found to be capable of accumulating the storage β-1,3-glucan in soluble form under heterotrophic conditions. In this study, the highest biomass yield of 32.8 g L was achieved by combining the utilization of ammonium chloride as the nitrogen source, simultaneous addition of vitamins B1 and B12 and maintenance of pH at 6.0. Sugar profiling and nuclear magnetic resonance analysis indicated that the P. malhamensis β-1,3-glucan was composed of glucose with the β-(1 → 3) main chain and the β-(1 → 6) side chain. Under the optimal cultivation conditions, the cellular β-1,3-glucan content was up to 55% of the cell dry weight. Moreover, the P. malhamensis β-1,3-glucan could significantly promote the fin regeneration and improve the in vivo antioxidative activity of zebrafish. This study underpins the feasibility of culturing P. malhamensis under heterotrophic conditions for producing the highly water-soluble bioactive β-1,3-glucans for food and pharmaceutical applications.
小球藻 Poterioochromonas malhamensis 被发现能够在异养条件下以可溶形式积累储存β-1,3-葡聚糖。在本研究中，通过组合利用氯化铵作为氮源、同时添加维生素 B1 和 B12 以及维持 pH 值在 6.0，实现了最高的 32.8 g/L 生物量产量。糖分析和核磁共振分析表明，P. malhamensis 的β-1,3-葡聚糖由葡萄糖组成，具有β-(1→3)主链和β-(1→6)侧链。在最佳培养条件下，细胞内β-1,3-葡聚糖含量高达细胞干重的 55%。此外，P. malhamensis 的β-1,3-葡聚糖可以显著促进斑马鱼的鳍再生，并提高其体内抗氧化活性。本研究支持了在异养条件下培养 P. malhamensis 生产高水溶性生物活性β-1,3-葡聚糖用于食品和制药应用的可行性。
