Division of Laboratory Animal Resources, Duke University Medical Center, Durham, North Carolina;, Email:
IDEXX BioAnalytics, Columbia, Missouri.
Comp Med. 2021 Aug 1;71(4):318-322. doi: 10.30802/AALAS-CM-21-000034. Epub 2021 Jun 29.
A small colony of zebrafish () experienced 30% acute mortality within a few days after receipt from a commercial source. A few fish presented with small areas of raised scales or tissue necrosis, primarily near the caudal peduncle. ( was identified by real-time PCR of pooled zebrafish and swabs of the pre-filter and fine filter pads, with subsequent sequence analysis. . is most commonly associated with an enteric septicemia in catfish species and can have significant economic impact on commercial catfish fisheries. However, several references report naturally occurring . infection of nonictalurid fishes, including zebrafish. Ours is the first report demonstrating the use of environmental sampling to identify in a zebrafish colony by real-time PCR. Moreover, our report indicates that . is a relevant disease for institutions using zebrafish as research species and emphasizes the importance of carefully considering importation and quarantine practices.
从商业来源收到几天后，一小群斑马鱼（）经历了 30%的急性死亡率。一些鱼出现了小面积的鳞片隆起或组织坏死，主要在尾柄附近。（通过对 pooled zebrafish 和预滤器和细滤垫拭子的实时 PCR 以及随后的序列分析进行鉴定。通常与鲶鱼类的肠败血症有关，并且对商业鲶鱼渔业有重大的经济影响。然而，有几个参考文献报告了非鲶鱼类中自然发生的感染，包括斑马鱼。我们的报告首次证明了使用环境采样通过实时 PCR 来鉴定斑马鱼群中的（。此外，我们的报告表明，对于使用斑马鱼作为研究物种的机构来说，是一种相关疾病，并强调了仔细考虑进口和检疫实践的重要性。
