1 Vitalant Research Institute, San Francisco, California.
2 Department of Laboratory Medicine, University of California, San Francisco, California.
Zebrafish. 2019 Jun;16(3):291-299. doi: 10.1089/zeb.2018.1710. Epub 2019 Apr 2.
Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20% in their P1 capsids and <36% in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of . Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56%) institutions tested. hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild-type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV-1 infection affects any immunological, developmental, or other biological processes in wild-type or mutant zebrafish lineages remains to be determined.
斑马鱼被广泛用作脊椎动物发育和病原体-宿主相互作用研究的模型系统。我们描述了一种新型微小核糖核酸病毒的完整基因组，该病毒是通过对斑马鱼肠道组织的病毒宏基因组分析鉴定的。该病毒与亲缘关系最近的病毒在 P1 衣壳上的同一性<20%，在 RdRp 上的同一性<36%，这表明斑马鱼微小核糖核酸病毒-1（ZfPV-1）是一个新属的成员，建议命名为 。对来自北美、欧洲和亚洲的斑马鱼进行逆转录（RT）-PCR 检测显示，ZfPV-1 分布广泛，在 41 个检测机构中的 23 个（56%）中检测到。 对整条斑马鱼的杂交显示，病毒 RNA 仅局限于肠的肠上皮细胞和下方固有层中的一组肠细胞和肠黏膜。这种自然发生的、显然无症状的感染（在野生型 AB 斑马鱼品系中）为研究微小核糖核酸病毒与宿主的相互作用提供了一个在高级脊椎动物模型生物中的天然感染系统。ZfPV-1 感染是否会影响野生型或突变型斑马鱼品系的任何免疫、发育或其他生物学过程，仍有待确定。
