Siddika Ayesha, Zahan Tasnim, Khatun Lipy, Habib Md Rowshanul, Aziz Md Abdul, Tareq A R M, Rahman Md Habibur, Karim Md Rezaul
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, 6205 Bangladesh.
Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment (AERE), Savar, Dhaka, 1349 Bangladesh.
Food Sci Biotechnol. 2020 Jun 22;29(9):1251-1260. doi: 10.1007/s10068-020-00775-x. eCollection 2020 Sep.
This study was designed to evaluate the antioxidant activity of methanol extract of Linn. leaves (MELA) using DPPH and ABTS free radical scavenging assays whereas its antineoplastic effect against Ehrlich ascites carcinoma (EAC) was assed using viable cell count, life span, body weight gain and hematological parameters of experimental mice. Results showed that rich phenolic and flavonoid content of MELA had moderate dose dependent free radical scavenging activity (IC: 62.0 μg/mL for DPPH and 6.0 μg/mL for ABTS). In vivo antineoplastic assay, MELA significantly ( < 0.05) decreased viable cells and body weight gain, increased the survival time and restored altered hematological profiles of cancer cell bearing mice. Fluorescence microscopic view of EAC cells derived from MELA-treated group showed apoptotic characteristics and this observation was also supported by overexpression of pro-apoptotic genes coding p53 and Bax proteins in treated cancer cells. The anti-apoptotic genes coding Bcl-2 protein was also absent in treated EAC cells as compared with the control. Moreover, phytochemical profiles of MELA as identified by GC/MS analysis are also consistent with its activities.
本研究旨在通过DPPH和ABTS自由基清除试验评估石蒜叶片甲醇提取物（MELA）的抗氧化活性，同时利用实验小鼠的活细胞计数、寿命、体重增加和血液学参数评估其对艾氏腹水癌（EAC）的抗肿瘤作用。结果表明，MELA中丰富的酚类和黄酮类成分具有中等剂量依赖性自由基清除活性（DPPH的IC：62.0μg/mL，ABTS的IC：6.0μg/mL）。在体内抗肿瘤试验中，MELA显著（<0.05）降低了活细胞数量和体重增加，延长了存活时间，并恢复了荷瘤小鼠改变的血液学指标。来自MELA处理组的EAC细胞的荧光显微镜观察显示出凋亡特征，并且在处理后的癌细胞中编码p53和Bax蛋白的促凋亡基因的过表达也支持了这一观察结果。与对照组相比，处理后的EAC细胞中也不存在编码Bcl-2蛋白的抗凋亡基因。此外，通过GC/MS分析鉴定的MELA的植物化学图谱也与其活性一致。
