Detection and characterization of refractory organic and inorganic pollutants discharged in biomethanated distillery effluent and their phytotoxicity, cytotoxicity, and genotoxicity assessment using Phaseolus aureus L. and Allium cepa L.

The color effluent discharged by alcohol distilleries comprises very high pollution loads due to the plethora of refractory chemicals even after anaerobic treatment and causing adverse effects to the environment. The present study aimed to examine the phytotoxic, cytotoxic, and genotoxic potential of the identified refractory organic and inorganic pollutants discharged in bio-methanated distillery effluent (BMDE). Physico-chemical analyses revealed that BMDE retains high BOD, COD, TDS along with heavy metals like Fe (572.64 mg L), Mn (4.269 mg L), Cd (1.631 mg L), Zn (2.547 mg L), Pb (1.262 mg L), (Cr 1.257 mg L), and Ni (0.781 mg L) beyond the permissible limits for effluent discharge. GC-MS analysis revelaed the presence of hexadecanoic acid, TMS ester; octadecanoic acid, TMS ester; 2,3 bis[(TMS)oxy]propyl ester; stigmasterol TMS ether; β-sitosterol TMS ester; hexacosanoic acid; and tetradecanoic acid, TMS ester as major refractory organic pollutants, which are listed as potential endocrine disruptor chemicals (EDCs) as per USEPA. Furthermore, phytotoxicity assessment with Phaseolus aureus L. showed the toxic nature of BMDE as it inhibited various seedling growth parameters, seed germination, and suppression of α-amylase activity in seed germination experiment. Moreover, genotoxicity and cytotoxicity evaluation of the discharged BMDE evidenced in root-tip meristematic cells of Allium cepa L. where chromosomal aberration such as disturbed metaphase, c-mitosis, laggard chromosomes, sticky chromosomes, prolonged prophase, polyploid cells, and apoptotic bodies etc. were observed. Thus, this study's results suggested that BMDE discharged without adequate treatment poses potential risk to environment and may cause a variety of serious health threats in living beings upon exposure.