Santolaya C, Hernández M R, Villaverde C A
Departamento de Farmacología y Patología Experimental, Centro de Investigación y Desarrollo de Barcelona, C.S.I.C., Spain.
Microcirc Endothelium Lymphatics. 1988 Jun;4(3):195-203.
Hemorheological changes produced in blood cells seem to be essential in atheroma plaque development and thrombotic episodes. In this study, we investigated the relationship between blood cells count, thrombogenic situations and morphological mesenteric alterations in atherosclerotic rats. Atherosclerosis was induced by an atherogenic diet made up of two phases, the first a hypervitaminic diet, and the second a hyperlipidic one. Cell counts were performed with Thoma's camera. Morphological changes were observed directly in rat mesentery. Thrombogenic situations were investigated by a mesenteric microthrombosis induction method. In atherosclerotic animals we can observe a higher mesenteric opacity, increase in blood viscosity and a thickness in vessel wall. Thrombosis time is shortened at 3 days, which indicates a thrombogenic situation although at 10 days there is a lenthening in this parameter. Blood cell counts were not modified significantly, but modifications in differential leukocyte counts were significant. We found a direct relationship between lymphocyte number and thrombosis time whereas with granulocytes this relationship was inverse: shortening in thrombosis time appearing simultaneously with an increase in cell number.
血细胞产生的血液流变学变化似乎在动脉粥样硬化斑块形成和血栓形成过程中至关重要。在本研究中，我们调查了动脉粥样硬化大鼠血细胞计数、血栓形成情况与肠系膜形态学改变之间的关系。动脉粥样硬化通过由两个阶段组成的致动脉粥样化饮食诱导，第一阶段是高维生素饮食，第二阶段是高脂饮食。使用托马氏血细胞计数器进行细胞计数。直接观察大鼠肠系膜的形态学变化。通过肠系膜微血栓形成诱导方法研究血栓形成情况。在动脉粥样硬化动物中，我们可以观察到肠系膜透明度更高、血液粘度增加以及血管壁增厚。在第3天血栓形成时间缩短，这表明存在血栓形成情况，尽管在第10天该参数有所延长。血细胞计数没有显著改变，但白细胞分类计数有显著变化。我们发现淋巴细胞数量与血栓形成时间之间存在直接关系，而与粒细胞的关系则相反：血栓形成时间缩短与细胞数量增加同时出现。
