Division of Infectious Diseases, University of Miami Miller School of Medicine, Miami, Florida, USA.
Department of Medicine, University of Miami Miller School of Medicine, Miami, Florida, USA.
J Med Virol. 2021 Oct;93(10):6059-6062. doi: 10.1002/jmv.27171. Epub 2021 Jul 6.
The utility of the polymerase chain reaction (PCR) cycle threshold (C ) values in the management of patients with coronavirus disease 2019 (COVID-19) remains controversial.
We assessed the correlation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) C values in nasopharyngeal swab samples with the oxygen requirements at the time of sample collection. Specimens were tested with the Simplexa PCR platform, which targets the SARS-CoV-2 ORF1ab and S genes.
We identified 23 COVID-19 patients with 49 C values available. While C values from ORF1ab and S genes were highly correlated for a given specimen, there was no correlation between C values for any of these target genes and the oxygen requirements of the patient at the time of sample collection. We found no differences in the initial nor the nadir C values between survivors and non-survivors or mild/moderate versus severe/critical illness at the maximum point of illness.
SARS-CoV-2 C values have limited value in the management of COVID-19.
聚合酶链反应（PCR）循环阈值（C）值在 2019 年冠状病毒病（COVID-19）患者管理中的应用仍然存在争议。
我们评估了鼻咽拭子样本中严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）C 值与采集样本时的氧气需求之间的相关性。标本采用 Simplexa PCR 平台检测，该平台针对 SARS-CoV-2 的 ORF1ab 和 S 基因。
我们确定了 23 例 COVID-19 患者，其中有 49 个 C 值可用。虽然特定标本的 ORF1ab 和 S 基因的 C 值高度相关，但这些靶基因中的任何一个的 C 值与采集样本时患者的氧气需求均无相关性。我们在疾病的最高点未发现幸存者和非幸存者之间、轻症/中度与重症/危重症之间的初始 C 值或最低 C 值存在差异。
SARS-CoV-2 的 C 值在 COVID-19 的管理中价值有限。
