Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, Shanxi, China.
Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, Shanxi, China.
Int J Biol Macromol. 2021 Aug 31;185:1022-1035. doi: 10.1016/j.ijbiomac.2021.06.160. Epub 2021 Jun 29.
Biochemical modification can endow the surface of implants with superior biological activity. Herein, silk fibroin (SF) protein and its anionic derivative peptides (Cs) were covalently immobilized onto a titanium implant surface via a polydopamine layer. The successful conjugation of SF and Cs was revealed by X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), and contact angle measurements. The addition of Cs prevented the conformational transition of silk fibroin to silk II. The deposition of apatite on its surface was significantly accelerated, and the bioactive composite coating was observed to enhance protein adsorption and cell proliferation. More importantly, it also promoted the osteogenic differentiation of bone marrow stem cells (BMSCs) for the quantitative and qualitative detection of alkaline phosphatase (ALP) and alizarin red (ARS). Overall, the stable performance and enhanced osteogenic property of the composite coating promote an extensive application for clinical titanium-based implants.
生化修饰可以赋予植入物表面优异的生物活性。在此，丝素蛋白（SF）及其阴离子衍生肽（Cs）通过聚多巴胺层共价固定在钛植入物表面。X 射线光电子能谱（XPS）、场发射扫描电子显微镜（FE-SEM）、原子力显微镜（AFM）和接触角测量揭示了 SF 和 Cs 的成功结合。Cs 的添加阻止了丝素蛋白向丝 II 的构象转变。其表面的磷灰石沉积明显加速，观察到生物活性复合涂层增强了蛋白质吸附和细胞增殖。更重要的是，它还促进了骨髓间充质干细胞（BMSCs）的成骨分化，用于碱性磷酸酶（ALP）和茜素红（ARS）的定量和定性检测。总的来说，复合涂层的稳定性能和增强的成骨特性促进了其在临床钛基植入物中的广泛应用。
