Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul 02447, Korea.
National Development Institute for Korean Medicine, Gyeongsan 38540, Korea.
Int J Mol Sci. 2022 Jul 27;23(15):8297. doi: 10.3390/ijms23158297.
Triterpenes are a diverse group of natural compounds found in plants. Soyasapogenol B (SoyB) from (peanut) has various pharmacological properties. This study aimed to elucidate the pharmacological properties and mechanisms of SoyB in bone-forming cells. In the present study, 1-20 μM of SoyB showed no cell proliferation effects, whereas 30-100 μM of SoyB increased cell proliferation in MC3T3-E1 cells. Next, osteoblast differentiation was analyzed, and it was found that SoyB enhanced ALP staining and activity and bone mineralization. SoyB also induced RUNX2 expression in the nucleus with the increased phosphorylation of Smad1/5/8 and JNK2 during osteoblast differentiation. In addition, SoyB-mediated osteoblast differentiation was not associated with autophagy and necroptosis. Furthermore, SoyB increased the rate of cell migration and adhesion with the upregulation of MMP13 levels during osteoblast differentiation. The findings of this study provide new evidence that SoyB possesses biological effects in bone-forming cells and suggest a potentially beneficial role for peanut-based foods.
三萜类化合物是植物中存在的一类多样化的天然化合物。来自 （花生）的大豆皂醇 B（SoyB）具有多种药理特性。本研究旨在阐明 SoyB 在成骨细胞中的药理特性和作用机制。在本研究中，1-20 μM 的 SoyB 对细胞增殖没有影响，而 30-100 μM 的 SoyB 则增加了 MC3T3-E1 细胞的增殖。接下来，分析了成骨细胞分化，发现 SoyB 增强了碱性磷酸酶染色和活性，并促进了骨矿化。SoyB 还诱导 RUNX2 在细胞核中的表达，同时在成骨细胞分化过程中增加 Smad1/5/8 和 JNK2 的磷酸化。此外，SoyB 介导的成骨细胞分化与自噬和坏死性凋亡无关。此外，SoyB 增加了细胞迁移和粘附率，并在成骨细胞分化过程中上调 MMP13 水平。本研究的结果提供了新的证据，表明 SoyB 在成骨细胞中具有生物学效应，并提示基于花生的食物可能具有有益作用。
