Adomaitė Gabija, Virbickas Povilas, Valiūnienė Aušra
Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.
Department of Biomedical Science, Faculty of Health and Society, Malmö University, SE-205 06 Malmö, Sweden.
Molecules. 2025 Jan 1;30(1):137. doi: 10.3390/molecules30010137.
This research presents a simple procedure for chemically modifying yeast () cells with nickel hexacyanoferrate (NiHCF) and ferric hexacyanoferrate, also known as Prussian blue (PB), to increase the conductivity of the yeast cell wall. Using linear sweep voltammetry, NiHCF-modified yeast and PB-modified yeast (NiHCF/yeast and PB/yeast, respectively) were found to have better cell wall conductivity in [Fe(CN)] and glucose-containing phosphate-buffered solution than unmodified yeast. Spectrophotometric analysis showed that the modification of yeast cells with NiHCF had a less harmful effect on yeast cell viability than the modification of yeast cells with PB. The use of NiHCF/yeast and PB/yeast cells in the construction of a yeast-based fuel cell allowed the maximum power densities of 62.66 mW/m and 94.09 mW/m to be achieved. These values were much higher than those obtained using unmodified yeast cells (42.25 mW/m). NiHCF/yeast and PB/yeast fuel cells were renewed by replenishing the yeast suspension in the anolyte or the FeCl salt in the catholyte. This allowed 77.4% and 50.1% of the initial maximum power density of the fuel cells to be achieved.
本研究提出了一种简单的程序，用六氰合铁酸镍（NiHCF）和六氰合铁酸铁（也称为普鲁士蓝（PB））对酵母（）细胞进行化学修饰，以提高酵母细胞壁的导电性。使用线性扫描伏安法发现，在含[Fe(CN)]和葡萄糖的磷酸盐缓冲溶液中，NiHCF修饰的酵母和PB修饰的酵母（分别为NiHCF/酵母和PB/酵母）比未修饰的酵母具有更好的细胞壁导电性。分光光度分析表明，与用PB修饰酵母细胞相比，用NiHCF修饰酵母细胞对酵母细胞活力的有害影响较小。在构建基于酵母的燃料电池中使用NiHCF/酵母和PB/酵母细胞，可实现的最大功率密度分别为62.66 mW/m和94.09 mW/m。这些值远高于使用未修饰酵母细胞获得的值（42.25 mW/m）。通过在阳极电解液中补充酵母悬浮液或在阴极电解液中补充FeCl盐来更新NiHCF/酵母和PB/酵母燃料电池。这使得燃料电池的初始最大功率密度分别达到77.4%和50.1%。
