Instituto de Neurobiología (INB), Universidad Nacional Autónoma de México (UNAM), Campus Juriquilla, Querétaro 76230, QT, Mexico.
Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana, No. 3918, Zona Playitas, Ensenada 22860, BC, Mexico.
Cells. 2021 Jun 23;10(7):1581. doi: 10.3390/cells10071581.
Cortical dysplasias are alterations in the organization of the layers of the brain cortex due to problems in neuronal migration during development. The neuronal component has been widely studied in experimental models of cortical dysplasias. In contrast, little is known about how glia are affected. In the cerebellum, Bergmann glia (BG) are essential for neuronal migration during development, and in adult they mediate the control of fine movements through glutamatergic transmission. The aim of this study was to characterize the morphology and intracellular calcium dynamics of BG and astrocytes from mouse cerebellum and their modifications in a model of cortical dysplasia induced by carmustine (BCNU). Carmustine-treated mice were affected in their motor coordination and balance. Cerebellar dysplasias and heterotopias were more frequently found in lobule X. Morphology of BG cells and astrocytes was affected, as were their spontaneous [Ca] transients in slice preparation and in vitro.
皮质发育不良是由于发育过程中神经元迁移出现问题导致的大脑皮层层结构的改变。神经元成分在皮质发育不良的实验模型中得到了广泛研究。相比之下，胶质细胞受到的影响却鲜为人知。在小脑，Bergmann 胶质细胞（BG）在发育过程中对神经元迁移至关重要，在成年期，它们通过谷氨酸能传递来控制精细运动。本研究的目的是描述来自小鼠小脑的 BG 和星形胶质细胞的形态和细胞内钙动力学及其在卡莫司汀（BCNU）诱导的皮质发育不良模型中的变化。卡莫司汀处理的小鼠在运动协调和平衡方面受到影响。小脑发育不良和异位在小叶 X 中更常见。BG 细胞和星形胶质细胞的形态受到影响，在切片制备和体外实验中，它们的自发[Ca]瞬变也受到影响。
