Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Viruses. 2021 Jun 23;13(7):1206. doi: 10.3390/v13071206.
Bacteriophages (phages) are a specific type of viruses that infect bacteria. Because of growing antibiotic resistance among bacterial strains, phage-based therapies are becoming more and more attractive. The critical problem is the storage of bacteriophages. Recently, it was found that bacteriophages might adsorb on the surfaces of plastic containers, effectively decreasing the titer of phage suspensions. Here, we showed that a BOA nanocomposite (gold nanoparticles embedded in polyoxoborate matrix) deposited onto the inner walls of the containers stabilizes phage suspensions against uncontrolled adsorption and titer decrease. Additionally, BOA provides antibacterial and antifungal protection. The application of BOA assures safe and sterile means for the storage of bacteriophages.
噬菌体（phages）是一种专门感染细菌的病毒。由于细菌菌株对抗生素的耐药性不断增加，基于噬菌体的治疗方法变得越来越有吸引力。关键问题是噬菌体的储存。最近发现，噬菌体可能会吸附在塑料容器的表面上，从而有效降低噬菌体悬浮液的效价。在这里，我们表明，沉积在容器内壁上的 BOA 纳米复合材料（金纳米颗粒嵌入多硼酸酯基质中）可稳定噬菌体悬浮液，防止不受控制的吸附和效价降低。此外，BOA 还提供了抗菌和抗真菌保护。BOA 的应用确保了噬菌体储存的安全和无菌手段。
