Gaowa Naren, Li Wenli, Gelsinger Sonia, Murphy Brianna, Li Shengli
College of Animal Science and Technology, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing 100193, China.
Cell Wall Biology and Utilization Research Unit, US Dairy Forage Research Center, Agricultural Research Service, US Department of Agriculture, 1925 Linden Drive, Madison, WI 53706, USA.
Metabolites. 2021 Jun 23;11(7):414. doi: 10.3390/metabo11070414.
Diet-induced acidosis imposes a health risk to young calves. In this study, we aimed to investigate the host jejunum transcriptome changes, along with its microbial community variations, using our established model of feed-induced ruminal acidosis in young calves. Eight bull calves were randomly assigned to two diet treatments beginning at birth (a starch-rich diet, Aci; a control diet, Con). Whole-transcriptome RNA sequencing was performed on the jejunum tissues collected at 17 weeks of age. Ribosomal RNA reads were used for studying microbial community structure variations in the jejunum. A total of 853 differentially expressed genes were identified (402 upregulated and 451 downregulated) between the two groups. The cell cycle and the digestion and absorption of protein in jejunal tissue were affected by acidosis. Compared to the control, genera of , and significantly increased in abundance in the Aci group, while and were significantly lower in the Aci group. Expression changes in the gene were associated with the abundance variations of a high number of genera in jejunum. Our study provided a snapshot of the transcriptome changes in the jejunum and its associated meta-transcriptome changes in microbial communities in young calves with feed-induced acidosis.
日粮诱导的酸中毒会给犊牛带来健康风险。在本研究中，我们旨在利用已建立的犊牛饲料诱导瘤胃酸中毒模型，研究宿主空肠转录组变化及其微生物群落变异。八头雄性犊牛从出生开始被随机分配到两种日粮处理组（富含淀粉的日粮，Aci；对照日粮，Con）。对17周龄时采集的空肠组织进行全转录组RNA测序。核糖体RNA读数用于研究空肠中微生物群落结构变异。两组之间共鉴定出853个差异表达基因（402个上调和451个下调）。酸中毒影响空肠组织中的细胞周期以及蛋白质的消化和吸收。与对照组相比，Aci组中、和属的丰度显著增加，而Aci组中的和显著降低。基因的表达变化与空肠中大量属的丰度变异有关。我们的研究提供了犊牛饲料诱导酸中毒时空肠转录组变化及其相关微生物群落元转录组变化的概况。
