Han Lulu, Tao Hui, Kang Lingyun, Wang Shuo, Diao Qiyu, Han Deping, Cui Kai
Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Front Vet Sci. 2022 Apr 25;9:809188. doi: 10.3389/fvets.2022.809188. eCollection 2022.
Early feeding regime has a substantial lifelong effect on lambs and weaning ewe's milk can lead to the intestinal injury of lambs. To explore the molecular regulatory mechanism of intestinal injury of lambs under weaning stress, the jejunum was conducted transcriptome and then integrated analyzed with our previous proteome data. A total of 255 upregulated genes and 285 downregulated genes were significantly identified. These genes showed low overlapping with differentially expressed proteins identified by isobaric tags for relative and absolute quantification (iTRAQ). However, according to their functions, the differentially expressed genes (DEGs) and proteins with the same expression trend were enriched for the similar Gene Ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as intestinal lipid absorption, urea cycle, peroxisome proliferator-activated receptor (PPAR) signaling pathway, and ferroptosis. Furthermore, the DEGs, including , and , might play essential roles in intestinal lipid absorption and immune response through the PPAR signaling pathway and ferroptosis. This study could provide new insights into early lamb breeding at the molecular level.
早期喂养方式对羔羊有重大的终生影响，断奶母羊的奶会导致羔羊肠道损伤。为了探究断奶应激下羔羊肠道损伤的分子调控机制，对空肠进行转录组分析，然后与我们之前的蛋白质组数据进行综合分析。共显著鉴定出255个上调基因和285个下调基因。这些基因与通过相对和绝对定量等压标签（iTRAQ）鉴定的差异表达蛋白的重叠度较低。然而，根据其功能，具有相同表达趋势的差异表达基因（DEGs）和蛋白质富集于相似的基因本体（GO）术语和京都基因与基因组百科全书（KEGG）通路，如肠道脂质吸收、尿素循环、过氧化物酶体增殖物激活受体（PPAR）信号通路和铁死亡。此外，包括 、 和 在内的DEGs可能通过PPAR信号通路和铁死亡在肠道脂质吸收和免疫反应中发挥重要作用。本研究可为早期羔羊育种在分子水平上提供新的见解。
