Zhong Zhiqiang, Zhang Yuning, Li Xiaotong, Li Lingyun, Zhang Ruiyang, Zhang Shuyi
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Animals (Basel). 2022 Jun 30;12(13):1695. doi: 10.3390/ani12131695.
In the present study, we utilized 16S rRNA sequencing to uncover the impacts of non-pelleted (HG) or high-grain pelleted (HP) diets on the microbial structure and potential functions of digesta- and mucosa-associated microbiota in the jejunum of Hu sheep. Here, we randomly assigned 15 healthy male Hu sheep into three groups and fed the control diets (CON), HG, and HP diets, respectively. The experiment period was 60 days. The HP diets had the same nutritional ingredients as the HG diets but in pelleted form. At the finish of the experiment, the jejunal digesta and mucosa were gathered for microbial sequencing. The results of PCoA and PERMANOVA showed that different dietary treatments had significant impact (p < 0.05) on digesta- and mucosa-associated microbiota in the jejunum of Hu sheep. For specific differences, HG diets significantly increased (p < 0.05) the abundance of some acid-producing bacteria in both jejunal digesta (Bifidobacterium, OTU151, and OTU16) and mucosa (Rikenellaceae RC9 gut group, and Bifidobacterium) of Hu sheep compared with the CON diets. Besides the similar effects of the HG diets (increased the acid-producing bacteria such as Olsenella, Pseudoramibacter, and Shuttleworthia), our results also showed that the HP diets significantly decreased (p < 0.05) the abundance of some pro-inflammatory bacteria in the jejunal digesta (Mogibacterium, and Marvinbryantia) and mucosa (Chitinophaga, and Candidatus Saccharimonas) of Hu sheep compared with the HG diets. Collectively, these findings contributed to enriching the knowledge about the effects of HG diets on the structure and function of intestinal microbiota in ruminants.
在本研究中，我们利用16S rRNA测序技术，揭示了非颗粒饲料（HG）或高谷物颗粒饲料（HP）对湖羊空肠中与食糜和黏膜相关的微生物群结构及潜在功能的影响。在此，我们将15只健康的雄性湖羊随机分为三组，分别饲喂对照日粮（CON）、HG日粮和HP日粮。实验期为60天。HP日粮与HG日粮具有相同的营养成分，但为颗粒形式。实验结束时，采集空肠食糜和黏膜进行微生物测序。主坐标分析（PCoA）和多变量方差分析（PERMANOVA）结果表明，不同的日粮处理对湖羊空肠中与食糜和黏膜相关的微生物群有显著影响（p < 0.05）。具体差异方面，与CON日粮相比，HG日粮显著增加（p < 0.05）了湖羊空肠食糜（双歧杆菌、OTU151和OTU16）和黏膜（理研菌科RC9肠道菌群和双歧杆菌）中一些产酸菌的丰度。除了HG日粮的类似效果（增加了产酸菌，如奥尔森氏菌、假瘤胃杆菌和沙氏菌属）外，我们的结果还表明，与HG日粮相比，HP日粮显著降低（p < 0.05）了湖羊空肠食糜（瘤胃杆菌属和马文氏菌属）和黏膜（噬几丁质菌属和暂定糖单胞菌属）中一些促炎菌的丰度。总的来说，这些发现有助于丰富关于HG日粮对反刍动物肠道微生物群结构和功能影响的知识。
