Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo 11382, Egypt.
Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11382, Egypt.
Int J Mol Sci. 2021 Jun 24;22(13):6770. doi: 10.3390/ijms22136770.
we aimed to construct a bioinformatics-based co-regulatory network of mRNAs and non coding RNAs (ncRNAs), which is implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), followed by its validation in a NAFLD animal model.
The mRNAs-miRNAs-lncRNAs regulatory network involved in NAFLD was retrieved and constructed utilizing bioinformatics tools. Then, we validated this network using an NAFLD animal model, high sucrose and high fat diet (HSHF)-fed rats. Finally, the expression level of the network players was assessed in the liver tissues using reverse transcriptase real-time polymerase chain reaction.
in-silico constructed network revealed six mRNAs ( and ), two miRNAs ( and ), and two lncRNAs (RPARP-AS1 and ) that play important roles as a co-regulatory network in NAFLD pathogenesis. Moreover, the expression level of these constructed network-players was significantly different between NAFLD and normal control. Conclusion and future perspectives: this study provides new insight into the molecular mechanism of NAFLD pathogenesis and valuable clues for the potential use of the constructed RNA network in effective diagnostic or management strategies of NAFLD.
构建一个与非酒精性脂肪性肝病（NAFLD）发病机制相关的基于生物信息学的 mRNA 和非编码 RNA（ncRNA）共调控网络，并在 NAFLD 动物模型中进行验证。
利用生物信息学工具检索并构建了与 NAFLD 相关的 mRNA-miRNA-lncRNA 调控网络。然后，我们使用 NAFLD 动物模型（高蔗糖和高脂肪饮食喂养的大鼠）验证了该网络。最后，使用逆转录实时聚合酶链反应评估网络参与者在肝组织中的表达水平。
计算机构建的网络揭示了六个 mRNAs（、、、、和）、两个 miRNAs（和）和两个 lncRNAs（RPARP-AS1 和）作为 NAFLD 发病机制中的共调控网络发挥着重要作用。此外，这些构建的网络参与者在 NAFLD 和正常对照组之间的表达水平存在显著差异。
本研究为 NAFLD 发病机制的分子机制提供了新的见解，并为构建的 RNA 网络在 NAFLD 的有效诊断或管理策略中的潜在应用提供了有价值的线索。
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