绞股蓝皂苷XLIX对体外脂肪肝细胞基因表达的影响：全基因组分析
Effects of Gypenoside XLIX on fatty liver cell gene expression in vitro: a genome-wide analysis.
作者信息
Liu Fengming, Wei Qiu, Liang Yidan, Yang Qingmei, Huang Chunxi, Huang Qiuju, Qin Jiankang, Pang Lili, Xu Liuyan, Zhong Juan
机构信息
Department of Critical Care Medicine, The First People's Hospital of Nanning Nanning 530022, Guangxi, China.
Department of Traditional Chinese Medicine, The First People's Hospital of Nanning Nanning 530022, Guangxi, China.
出版信息
Am J Transl Res. 2023 Feb 15;15(2):834-846. eCollection 2023.
OBJECTIVE
To perform Genome-wide analysis of Gypenoside XLIX (Gyp-XLIX) in the treatment of fatty liver cells.
METHODS
The gene profiles of 3 normal liver cells, 3 fatty liver cells, and 3 fatty liver cells treated with Gyp-XLIX were detected by high-throughput sequencing to identify the differentially expressed genes (DEGs) in fatty liver treated by Gyp-XLIX. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were used to explore the biological functions of DEGs. By constructing lncRNA-mRNA co-expression network of DEGs, network node genes were mined. Possible target genes of differentially expressed lncRNA were predicted by cis regulation.
RESULTS
782 DEGs were screened out; that is, 172 genes were highly expressed in fatty liver cells, and the expression decreased to the level of normal liver cells after Gyp-XLIX treatment; 610 genes were under expressed in fatty liver cells, and the expression increased to the level of normal liver cells after Gyp-XLIX treatment. Functional analysis of KEGG and GO showed that DEGs process DNA-binding transcription factor activity and ion transmembrane transporter activity in the plasma membrane region. This mediates glycerophospholipid metabolism, bile secretion, fatty acid degradation and other signaling pathways. lncRNA analysis showed that the expression of 16 lncRNAs was low in fatty liver cells, and the expression was increased to the level of normal liver cells after Gyp-XLIX treatment. Target gene prediction showed that 16 differentially expressed lncRNAs had cis potential to regulate target genes, among which lncRNA RPARP-AS1 had a high degree of relationship with other genes. lncRNA-mRNA co-expression network results showed that lncRNA RPARP-AS1 may acted on .
CONCLUSION
LncRNA was differentially expressed in fatty liver cells and Gyp-XLIX treated fatty liver cells, and lncRNA RPARP-AS1 may be a regulatory gene in Gyp-XLIX treated fatty liver.
目的
对绞股蓝总苷XLIX（Gyp-XLIX）治疗脂肪肝细胞进行全基因组分析。
方法
通过高通量测序检测3个正常肝细胞、3个脂肪肝细胞和3个经Gyp-XLIX处理的脂肪肝细胞的基因谱，以鉴定Gyp-XLIX处理的脂肪肝中差异表达基因（DEG）。采用基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析来探索DEG的生物学功能。通过构建DEG的lncRNA-mRNA共表达网络，挖掘网络节点基因。通过顺式调控预测差异表达lncRNA的可能靶基因。
结果
筛选出782个DEG；即172个基因在脂肪肝细胞中高表达，经Gyp-XLIX处理后表达降至正常肝细胞水平；610个基因在脂肪肝细胞中低表达，经Gyp-XLIX处理后表达升至正常肝细胞水平。KEGG和GO功能分析表明，DEG在质膜区域具有DNA结合转录因子活性和离子跨膜转运活性。这介导了甘油磷脂代谢、胆汁分泌、脂肪酸降解等信号通路。lncRNA分析表明，16种lncRNA在脂肪肝细胞中表达较低，经Gyp-XLIX处理后表达升至正常肝细胞水平。靶基因预测表明，16种差异表达的lncRNA具有顺式调控靶基因的潜力，其中lncRNA RPARP-AS1与其他基因的关系高度相关。lncRNA-mRNA共表达网络结果表明lncRNA RPARP-AS1可能作用于……
结论
lncRNA在脂肪肝细胞和经Gyp-XLIX处理的脂肪肝细胞中差异表达，lncRNA RPARP-AS1可能是Gyp-XLIX处理脂肪肝中的一个调控基因。
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