Department of Botany, Faculty of Biology, Sofia University, 8 blvd. Dragan Zankov, 1164 Sofia, Bulgaria.
AgroBioInstitute, Bulgarian Agricultural Academy, 8 blvd. Dragan Zankov, 1164 Sofia, Bulgaria.
Toxins (Basel). 2021 Jun 28;13(7):448. doi: 10.3390/toxins13070448.
Bulgaria, situated on the Balkan Peninsula, is rich in small and shallow, natural and man-made non-lotic waterbodies, which are threatened by blooms of Cyanoprokaryota/Cyanobacteria. Although cyanotoxins in Bulgarian surface waters are receiving increased attention, there is no information on microviridins and their producers. This paper presents results from a phytoplankton study, conducted in August 2019 in three lakes (Durankulak, Vaya, Uzungeren) and five reservoirs (Duvanli, Mandra, Poroy, Sinyata Reka, Zhrebchevo) in which a molecular-genetic analysis (PCR based on the precursor gene and subsequent translation to amino acid alignments), combined with conventional light microscopy and an HPLC analysis of marker pigments, were applied for the identification of potential microviridin producers. The results provide evidence that ten strains of the genus , and of its most widespread species in particular, are potentially toxigenic in respect to microviridins. The sequences were obtained from all studied waterbodies and their translation to amino-acid alignments revealed the presence of five microviridin variants (types B/C, Izancya, CBJ55500.1 ( 199), and MC19, as well as a variant, which was very close to type A). This study adds to the general understanding of the microviridin occurrence, producers, and sequence diversity.
保加利亚位于巴尔干半岛，拥有丰富的小型浅淡水体，包括自然和人工的非流态水体，这些水体受到蓝藻/蓝细菌水华的威胁。尽管保加利亚地表水的氰基原核生物/蓝藻毒素受到越来越多的关注，但有关微绿菌素及其产生菌的信息却很少。本文介绍了 2019 年 8 月在三个湖泊（杜兰库拉克、瓦亚、乌宗热伦）和五个水库（杜万利、曼德拉、波洛伊、辛亚特雷卡、日布切沃）进行的浮游植物研究结果，该研究采用了分子遗传学分析（基于前体基因的 PCR 及随后的氨基酸比对翻译），结合常规光镜和标记色素的 HPLC 分析，用于鉴定潜在的微绿菌素产生菌。结果表明，属的十种菌株，特别是其分布最广泛的种 ，在微绿菌素方面具有潜在的产毒能力。从所有研究水体中获得了 序列，并将其翻译为氨基酸比对，揭示了存在五种微绿菌素变体（B/C 型、Izancya、CBJ55500.1（199）和 MC19，以及一种非常接近 A 型的变体）。本研究增加了对微绿菌素发生、产生菌和序列多样性的总体认识。
