Faculty of Biology, Department of Botany, Sofia University, BG-1164 Sofia, Bulgaria.
AgroBio Institute, Bulgarian Agricultural Academy, BG-1164 Sofia, Bulgaria.
Toxins (Basel). 2022 Nov 10;14(11):778. doi: 10.3390/toxins14110778.
The paper presents the first proof of planktonic cyanoprokaryote genus as an anatoxin A (ATX) producer in Bulgarian wetlands. The results from polymerase chain reaction (PCR) obtained from two summer sampling campaigns in 26 selected lakes and reservoirs demonstrated presence of the gene, responsible for ATX production in 21 strains of the genus. They were found in three waterbodies sampled in 2018 (coastal lake Vaya, coastal reservoir Poroy, inland reservoir Sinyata Reka) and in four waterbodies sampled in 2019 (inland reservoirs Duvanli, Koprinka, Plachidol 2, Sinyata Reka). The detected genetic diversity generally corresponds to the observations conducted by conventional light microscopy, by which we distinguished three species of (, and , the latter considered alien in the country). Eleven strains showed high similarity to two sequences of available from the National Centre for Biotechnology Information (NCBI). Ten other strains assembled in a group, which-in lack of available from NCBI genetic sequences-were presumed related to and after comparison with the results from light microscopy. strains found in Bulgarian waterbodies showed high genetic similarity to those isolated and sequenced from Asia (Japan, China) and Northern Europe (Norway, Finland).
本文首次证明浮游蓝藻门 属能够产生anatoxin A (ATX)。从 26 个选定湖泊和水库的两个夏季采样活动中获得的聚合酶链反应 (PCR) 结果表明，有 21 株属的基因存在，负责 ATX 的产生。它们存在于 2018 年采样的三个水体中（沿海湖泊 Vaya、沿海水库 Poroy、内陆水库 Sinyata Reka）和 2019 年采样的四个水体中（内陆水库 Duvanli、Koprinka、Plachidol 2、Sinyata Reka）。检测到的遗传多样性通常与通过常规光镜观察到的结果相对应，通过光镜观察，我们区分了三种 属的物种（、和 ，后一种被认为是该国的外来物种）。有 11 株菌与来自国家生物技术信息中心 (NCBI) 的两个可用序列具有高度相似性。其他 10 株菌在一个群体中聚集在一起，由于缺乏 NCBI 遗传序列，在与光镜观察结果进行比较后，这些菌株被认为与 和 有关。在保加利亚水体中发现的 菌株与从亚洲（日本、中国）和北欧（挪威、芬兰）分离和测序的菌株具有高度的遗传相似性。
