Brunet Camille D, Hennebique Aurélie, Peyroux Julien, Pelloux Isabelle, Caspar Yvan, Maurin Max
Centre National de la Recherche Scientifique, Université Grenoble Alpes, TIMC, UMR5525, 38000 Grenoble, France.
Centre National de Référence des Francisella, Centre Hospitalier Universitaire Grenoble Alpes, 38000 Grenoble, France.
Microorganisms. 2021 Jun 28;9(7):1398. doi: 10.3390/microorganisms9071398.
In 2018, the incidence of tularemia increased twofold in the west of France, with many pneumonic forms, suggesting environmental sources of infection. We investigated the presence of subsp. and other species DNA in the natural aquatic environment of this geographic area. Two sampling campaigns, in July 2019 and January 2020, allowed the collection of 87 water samples. Using a combination of real-time PCR assays, we tested the presence of either sp., and subsp. , the latter being the only tularemia agent in Europe. Among 57 water samples of the first campaign, 15 (26.3%) were positive for sp., nine (15.8%) for and/or , and four (7.0%) for subsp. . Ratios were 25/30 (83.3%), 24/30 (80.0%), and 4/30 (13.3%) for the second campaign. Among the thirty sites sampled during the two campaigns, nine were positive both times for sp., seven for and/or , and one for subsp. . Altogether, our study reveals a high prevalence of sp. DNA (including the tularemia agent) in the studied aquatic environment. This aquatic environment could therefore participate in the endemicity of tularemia in the west of France.
2018年，法国西部兔热病的发病率增长了两倍，出现了许多肺型病例，提示存在环境感染源。我们调查了该地理区域自然水生环境中兔热弗朗西斯菌亚种及其他弗朗西斯菌属物种DNA的存在情况。在2019年7月和2020年1月开展的两次采样活动中，共采集了87份水样。我们使用实时聚合酶链反应检测组合，检测了土拉热弗朗西斯菌、新凶手弗朗西斯菌和兔热弗朗西斯菌亚种（后者是欧洲唯一的兔热病病原体）的存在情况。在第一次采样活动的57份水样中，15份（26.3%）土拉热弗朗西斯菌检测呈阳性，9份（15.8%）新凶手弗朗西斯菌和/或土拉热弗朗西斯菌检测呈阳性，4份（7.0%）兔热弗朗西斯菌亚种检测呈阳性。第二次采样活动的相应比例分别为25/30（83.3%）、24/30（80.0%）和4/30（13.3%）。在两次采样活动中均进行采样的30个地点中，9个地点两次土拉热弗朗西斯菌检测均呈阳性，7个地点新凶手弗朗西斯菌和/或土拉热弗朗西斯菌检测呈阳性，1个地点兔热弗朗西斯菌亚种检测呈阳性。总体而言，我们的研究揭示了所研究的水生环境中土拉热弗朗西斯菌DNA（包括兔热病病原体）的高流行率。因此，这种水生环境可能与法国西部兔热病的地方性流行有关。
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