Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
Department of Chemistry Education, Seoul National University, Seoul 05029, Korea.
Sensors (Basel). 2021 Jun 15;21(12):4099. doi: 10.3390/s21124099.
Prostate-specific antigen (PSA) is the best-known biomarker for early diagnosis of prostate cancer. For prostate cancer in particular, the threshold level of PSA <4.0 ng/mL in clinical samples is an important indicator. Quick and easy visual detection of the PSA level greatly helps in early detection and treatment of prostate cancer and reducing mortality. In this study, we developed optimized silica-coated silver-assembled silica nanoparticles (SiO@Ag@SiO NPs) that were applied to a visual lateral flow immunoassay (LFIA) platform for PSA detection. During synthesis, the ratio of silica NPs to silver nitrate changed, and as the synthesized NPs exhibited distinct UV spectra and colors, most optimized SiO@Ag@SiO NPs showed the potential for early prostate cancer diagnosis. The PSA detection limit of our LFIA platform was 1.1 ng/mL. By applying each SiO@Ag@SiO NP to the visual LFIA platform, optimized SiO@Ag@SiO NPs were selected in the test strip, and clinical samples from prostate cancer patients were successfully detected as the boundaries of non-specific binding were clearly seen and the level of PSA was <4 ng/mL, thus providing an avenue for quick prostate cancer diagnosis and early treatment.
前列腺特异性抗原（PSA）是早期诊断前列腺癌的最著名的生物标志物。对于前列腺癌来说，临床样本中 PSA<4.0ng/mL 的阈值水平是一个重要指标。快速、简便的 PSA 水平目视检测极大地有助于前列腺癌的早期发现和治疗，降低死亡率。在这项研究中，我们开发了优化的二氧化硅涂层银组装二氧化硅纳米粒子（SiO@Ag@SiO NPs），并将其应用于用于 PSA 检测的可视化侧向流动免疫分析（LFIA）平台。在合成过程中，改变了二氧化硅纳米粒子与硝酸银的比例，由于合成的 NPs 表现出明显的紫外光谱和颜色，大多数优化的 SiO@Ag@SiO NPs 显示出早期前列腺癌诊断的潜力。我们的 LFIA 平台的 PSA 检测限为 1.1ng/mL。通过将每个 SiO@Ag@SiO NP 应用于可视化 LFIA 平台，在测试条上选择了优化的 SiO@Ag@SiO NPs，并且成功地检测到来自前列腺癌患者的临床样本，因为可以清楚地看到非特异性结合的边界，并且 PSA 水平<4ng/mL，从而为快速诊断前列腺癌和早期治疗提供了途径。
