Bock Sungje, Kim Hyung-Mo, Kim Jaehi, An Jaehyun, Choi Yun-Sik, Pham Xuan-Hung, Jo Ahla, Ham Kyeong-Min, Song Hobeom, Kim Jung-Won, Hahm Eunil, Rho Won-Yeop, Lee Sang Hun, Park Seung-Min, Lee Sangchul, Jeong Dae Hong, Lee Ho-Young, Jun Bong-Hyun
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
BioSquare Inc., Hwaseong 18449, Korea.
Nanomaterials (Basel). 2021 Dec 23;12(1):33. doi: 10.3390/nano12010033.
Prostate cancer can be detected early by testing the presence of prostate-specific antigen (PSA) in the blood. Lateral flow immunoassay (LFIA) has been used because it is cost effective and easy to use and also has a rapid sample-to-answer process. Quantum dots (QDs) with very bright fluorescence have been previously used to improve the detection sensitivity of LFIAs. In the current study, a highly sensitive LFIA kit was devised using QD-embedded silica nanoparticles. In the present study, only a smartphone and a computer software program, ImageJ, were used, because the developed system had high sensitivity by using very bright nanoprobes. The limit of PSA detection of the developed LFIA system was 0.138 ng/mL. The area under the curve of this system was calculated as 0.852. The system did not show any false-negative result when 47 human serum samples were analyzed; it only detected PSA and did not detect alpha-fetoprotein and newborn calf serum in the samples. Additionally, fluorescence was maintained on the strip for 10 d after the test. With its high sensitivity and convenience, the devised LFIA kit can be used for the diagnosis of prostate cancer.
通过检测血液中前列腺特异性抗原（PSA）的存在，可以早期发现前列腺癌。已使用侧向流动免疫分析（LFIA），因为它具有成本效益且易于使用，并且还具有快速的样本到答案过程。具有非常明亮荧光的量子点（QD）先前已用于提高LFIAs的检测灵敏度。在当前研究中，设计了一种使用嵌入量子点的二氧化硅纳米颗粒的高灵敏度LFIA试剂盒。在本研究中，仅使用了智能手机和计算机软件程序ImageJ，因为所开发的系统通过使用非常明亮的纳米探针具有高灵敏度。所开发的LFIA系统的PSA检测限为0.138 ng/mL。该系统的曲线下面积计算为0.852。在分析47份人血清样本时，该系统未显示任何假阴性结果；它仅检测到PSA，未检测到样本中的甲胎蛋白和新生小牛血清。此外，测试后条带上的荧光保持10天。所设计的LFIA试剂盒具有高灵敏度和便利性，可用于前列腺癌的诊断。
