Kumari Sangeeta, Phogat Deepak, Sehrawat Krishnan D, Choudhary Ravish, Rajput Vishnu D, Ahlawat Jyoti, Karunakaran Rohini, Minkina Tatiana, Sehrawat Anita R
Department of Botany, Maharshi Dayanand University, Rohtak 124001, India.
Sarvodya College of Pharmacy, Imlota 127306, India.
Plants (Basel). 2021 Jun 15;10(6):1216. doi: 10.3390/plants10061216.
Mung bean ( L.) sprout is a popular fresh vegetable, tasty and high in antioxidants. To increase yield and quality after the occurrence of both abiotic and biotic stresses, the application of seaweed extracts is of great importance. Hence, this study was conducted to determine the effect of extract (ANE) in the presence of salt on the antioxidant potential of sprouts. Different concentrations of ANE viz. 0.00, 0.01, 0.05, 0.10, and 0.50% and NaCl 0, 25, 50, 75, and 100 mM alone and in combinations were tested for researching the antioxidant potential of sprouts at 0, 24, and 36 h of sprouting. The DPPH free-radical-scavenging activity of sprouts of was found to increase with time and peaked at 24 h of treatment. The extract (0.01%) could reverse the ill effect of the low level of salinity posed by up to 25 mM NaCl. The increasing salinity deteriorated the antioxidant activity using ABTS method of sprouts down to 20.45% of the control at 100 mM NaCl. The total phenolic content (TPC), total flavonoid content (TFC), and reducing power of sprouts was found to increase till 36 h of sprouting. A slight increase in TPC, TFC and reducing power was observed when seeds were treated with low concentrations of ANE. The elevation in TPC, TFC and reducing power upon treatment with low concentrations of ANE was also noticed in sprouts in saline combinations. Alpha amylase inhibition activity was found to reach a (67.16% ± 0.9) maximum at 24 h of sprouting at a 0.01% concentration of ANE. Tyrosinase inhibition and alpha glucosidase inhibition was 88.0% ± 2.11 and 84.92% ± 1.2 at 36 h of sprouting, respectively, at 0.01% concentration of ANE. extract is natural, environmentally friendly, and safe, and could be used as one of the strategies to decline stress at a low level and enhance the antioxidant activities in sprouts, thus increasing its potential to be developed as an antioxidant-based functional food.
绿豆(Vigna radiata (L.))芽苗菜是一种广受欢迎的新鲜蔬菜,味道鲜美且富含抗氧化剂。为了在非生物和生物胁迫发生后提高产量和品质,施用海藻提取物非常重要。因此,本研究旨在确定盐胁迫下海藻提取物(ANE)对绿豆芽苗菜抗氧化潜力的影响。分别测试了不同浓度的ANE,即0.00%、0.01%、0.05%、0.10%和0.50%,以及单独和组合使用的0、25、50、75和100 mM的NaCl,以研究发芽0、24和36小时时绿豆芽苗菜的抗氧化潜力。发现绿豆芽苗菜的DPPH自由基清除活性随时间增加,并在处理24小时时达到峰值。0.01%的ANE提取物可以逆转高达25 mM NaCl造成的低水平盐胁迫的不良影响。使用ABTS法,随着盐浓度增加,绿豆芽苗菜的抗氧化活性下降,在100 mM NaCl时降至对照的20.45%。发现绿豆芽苗菜的总酚含量(TPC)、总黄酮含量(TFC)和还原能力在发芽36小时前一直增加。当种子用低浓度的ANE处理时,观察到TPC、TFC和还原能力略有增加。在盐胁迫组合的绿豆芽苗菜中,用低浓度ANE处理后TPC、TFC和还原能力也有所提高。发现淀粉酶抑制活性在发芽24小时、ANE浓度为0.01%时达到最大值(67.16%±0.9)。在发芽36小时、ANE浓度为0.01%时,酪氨酸酶抑制率和α-葡萄糖苷酶抑制率分别为88.0%±2.11和84.92%±1.2。ANE提取物天然、环保且安全,可作为降低低水平胁迫和增强绿豆芽苗菜抗氧化活性的策略之一,从而提高其作为基于抗氧化剂的功能性食品的开发潜力。