Fozi Vahid, Esmaeili Hassan, Alizadeh Abouzar, Eghlima Ghasem, Mirjalili Mohammad Hossein
Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
Faculty of Agriculture, Lorestan University, Khorramabad, Lorestan, Iran.
Front Plant Sci. 2024 Oct 7;15:1474399. doi: 10.3389/fpls.2024.1474399. eCollection 2024.
With increasing drought stress due to climate change and water scarcity, the agricultural sector has sought innovative strategies to mitigate the detrimental effects on crop productivity. One approach that has received significant attention is the use of fertilizers and biostimulants as potential means of alleviating drought stress.
In this study, five different irrigation levels including 100% (control), 80% (slight stress), 60% (mild stress), 40% (moderate stress), and 20% (severe stress) of field capacity (FC) and seaweed extract (SWE) at three concentrations (0, 5, and 10 g/L) were applied to the pots containing one-year-old licorice () plants in a factorial completely randomized design experiment with three replications for eight weeks.
The glycyrrhizic acid content increased with water stress intensity without the application of SWE until severe (20% FC) water stress treatment. The application of 10 g/L SWE under 100% FC led to a significant increase in the glycyrrhizic acid value (32.5±0.889 mg/g DW) compared with non-SWE application (30.0±1.040 mg/g DW). The maximum glabridin content (0.270±0.010 mg/g DW) was obtained under irrigation of 20% field capacity with 10 g/L SWE application. In addition, the activity of the all studied enzymes such as APX (ascorbate peroxidase), CAT (catalase), POD (peroxidase), and SOD (superoxide dismutase) were boosted by increasing the water stress levels. The use of SWE further enhanced the increase of some of these metabolites and enzymes, which, in turn, helped the plant to tolerate stress conditions through the scavenging of more ROS (Reactive oxygen species), wherein for this purpose, the SWE 10 g/L was more effective than other concentration. The plants efficiently eliminated ROS driven from drought stress by both non-enzymatic and enzymatic systems.
随着气候变化和水资源短缺导致干旱胁迫加剧，农业部门一直在寻求创新策略来减轻对作物生产力的不利影响。一种受到广泛关注的方法是使用肥料和生物刺激剂作为缓解干旱胁迫的潜在手段。
在本研究中，采用析因完全随机设计实验，将五种不同的灌溉水平（包括田间持水量（FC）的100%（对照）、80%（轻度胁迫）、60%（中度胁迫）、40%（重度胁迫）和20%（极重度胁迫））和三种浓度（0、5和10 g/L）的海藻提取物（SWE）施用于装有一年生甘草植株的花盆中，重复三次，为期八周。
在不施用SWE的情况下，直到极重度（20% FC）水分胁迫处理，甘草酸含量随水分胁迫强度增加而增加。在100% FC条件下施用10 g/L SWE导致甘草酸值显著增加（32.5±0.889 mg/g DW），相比之下未施用SWE时为（30.0±1.040 mg/g DW）。在20%田间持水量灌溉并施用10 g/L SWE的条件下，获得了最高的光甘草定含量（0.270±0.010 mg/g DW）。此外，随着水分胁迫水平的增加，所有研究的酶如抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）、过氧化物酶（POD）和超氧化物歧化酶（SOD）的活性均增强。SWE的使用进一步增强了其中一些代谢物和酶的增加，这反过来又通过清除更多的活性氧（ROS）帮助植物耐受胁迫条件，为此，10 g/L的SWE比其他浓度更有效。植物通过非酶和酶系统有效地消除了干旱胁迫产生的ROS。
