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……的酚类成分、酶抑制作用及抗氧化活性

Phenolic composition, enzyme inhibitory, and antioxidant activity of .

作者信息

Sarikurkcu Cengiz, Cengiz Mustafa, Uren Mehmet Cemil, Ceylan Olcay, Orenc Tuba, Tepe Bektas

机构信息

1Faculty of Pharmacy, Department of Analytical Chemistry, Süleyman Demirel University, Isparta, Turkey.

2Faculty of Science and Literature, Department of Chemistry, Süleyman Demirel University, Isparta, Turkey.

出版信息

Food Sci Biotechnol. 2016 Oct 31;25(5):1299-1304. doi: 10.1007/s10068-016-0204-6. eCollection 2016.

Abstract

This study aimed to evaluate the antioxidant and enzyme inhibitory activities of ethyl acetate, methanol, and water extracts of . In phosphomolybdenum assay, the methanol extract showed the highest activity (166.78 μmol TEs/g dry plant). The water extract exhibited the highest scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazloine-6-sulphonic acid) (ABTS). In addition, it exhibited the highest activity in cupric ion reducing (CUPRAC) and ferric reducing antioxidant power (FRAP) assays (41.26 and 46.82 μmol TEs/g dry plant). The extracts did not show cholinesterase and tyrosinase inhibitory activity. However, α-glucosidase inhibition assay resulted in the superiority of water extract (1233.86 μmol ACEs/g dry plant). In the case of α-amylase inhibitory assay, the ethyl acetate extract showed the highest activity (53.65 μmol ACEs/g dry plant). The water extract exhibited the highest phenolic content (31.70 μmol GAEs/g dry plant). In contrast, the methanol extract was found rich in flavonoid compounds (5.29 μmol REs/g dry plant). The water extract contained considerable amounts of rosmarinic acid, luteolin, quercetin, and rutin. Therefore, it can be used as a source of new and alternative antioxidant and enzyme inhibitory agents.

摘要

本研究旨在评估[植物名称]的乙酸乙酯、甲醇和水提取物的抗氧化及酶抑制活性。在磷钼酸测定中,甲醇提取物显示出最高活性(166.78 μmol 没食子酸当量/g干植物)。水提取物对1,1 - 二苯基 - 2 - 苦基肼(DPPH)和2,2 - 联氮 - 双 -(3 - 乙基苯并噻唑啉 - 6 - 磺酸)(ABTS)表现出最高的清除活性。此外,在铜离子还原(CUPRAC)和铁离子还原抗氧化能力(FRAP)测定中它也表现出最高活性(41.26和46.82 μmol没食子酸当量/g干植物)。这些提取物未显示出胆碱酯酶和酪氨酸酶抑制活性。然而,α - 葡萄糖苷酶抑制试验显示水提取物具有优势(1233.86 μmol 乙酰胆碱酯酶当量/g干植物)。在α - 淀粉酶抑制试验中,乙酸乙酯提取物显示出最高活性(53.65 μmol 乙酰胆碱酯酶当量/g干植物)。水提取物表现出最高的酚类含量(31.70 μmol 没食子酸当量/g干植物)。相比之下,甲醇提取物富含黄酮类化合物(5.29 μmol 芦丁当量/g干植物)。水提取物含有大量的迷迭香酸、木犀草素、槲皮素和芦丁。因此,它可作为新型替代抗氧化剂和酶抑制剂的来源。

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