Department of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, Hail P.O. Box 2440, Saudi Arabia.
Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia.
Molecules. 2021 Jun 12;26(12):3609. doi: 10.3390/molecules26123609.
(Okra) is an important vegetable crop, widely cultivated around the world due to its high nutritional significance along with several health benefits. Different parts of okra including its mucilage have been currently studied for its role in various therapeutic applications. Therefore, we aimed to develop and characterize the okra mucilage biopolymer (OMB) for its physicochemical properties as well as to evaluate its in vitro antidiabetic activity. The characterization of OMB using Fourier-transform infrared spectroscopy (FT-IR) revealed that okra mucilage containing polysaccharides lies in the bandwidth of 3279 and 1030 cm, which constitutes the fingerprint region of the spectrum. In addition, physicochemical parameters such as percentage yield, percentage solubility, and swelling index were found to be 2.66%, 96.9%, and 5, respectively. A mineral analysis of newly developed biopolymers showed a substantial amount of calcium (412 mg/100 g), potassium (418 mg/100 g), phosphorus (60 mg/100 g), iron (47 mg/100 g), zinc (16 mg/100 g), and sodium (9 mg/100 g). The significant antidiabetic potential of OMB was demonstrated using α-amylase and α-glucosidase enzyme inhibitory assay. Further investigations are required to explore the newly developed biopolymer for its toxicity, efficacy, and its possible utilization in food, nutraceutical, as well as pharmaceutical industries.
(秋葵)是一种重要的蔬菜作物,由于其高营养价值以及对健康的多种益处,在世界各地广泛种植。秋葵的不同部位,包括其黏液,目前正因其在各种治疗应用中的作用而受到研究。因此,我们旨在开发和表征秋葵黏液生物聚合物(OMB),以研究其物理化学特性,并评估其体外抗糖尿病活性。使用傅里叶变换红外光谱(FT-IR)对 OMB 的表征表明,含有多糖的秋葵黏液位于 3279 和 1030 cm 的带宽内,这构成了光谱的指纹区域。此外,发现物理化学参数,如产率百分比、溶解度百分比和溶胀指数分别为 2.66%、96.9%和 5。对新开发的生物聚合物的矿物质分析表明,含有大量的钙(412 mg/100 g)、钾(418 mg/100 g)、磷(60 mg/100 g)、铁(47 mg/100 g)、锌(16 mg/100 g)和钠(9 mg/100 g)。使用α-淀粉酶和α-葡萄糖苷酶抑制试验证明了 OMB 的显著抗糖尿病潜力。需要进一步的研究来探索新开发的生物聚合物的毒性、功效及其在食品、营养保健品和制药行业中的可能利用。