Raveau Robin, Lounès-Hadj Sahraoui Anissa, Hijri Mohamed, Fontaine Joël
Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, UR 4492, SFR Condorcet FR CNRS 3417, 50 rue Ferdinand Buisson, CEDEX, 62228 Calais, France.
Institut de Recherche en Biologie Végétale (IRBV) de l'Université de Montréal, 4101 Rue Sherbrooke E, Montréal, QC H1X2B2, Canada.
Microorganisms. 2021 Jun 19;9(6):1333. doi: 10.3390/microorganisms9061333.
Soil fungal communities play a central role in natural systems and agroecosystems. As such, they have attracted significant research interest. However, the fungal microbiota of aromatic plants, such as clary sage ( L.), remain unexplored. This is especially the case in trace element (TE)-polluted conditions and within the framework of phytomanagement approaches. The presence of high concentrations of TEs in soils can negatively affect not only microbial diversity and community composition but also plant establishment and growth. Hence, the objective of this study is to investigate the soil fungal and arbuscular mycorrhizal fungi (AMF) community composition and their changes over time in TE-polluted soils in the vicinity of a former lead smelter and under the cultivation of clary sage. We used Illumina MiSeq amplicon sequencing to evaluate the effects of in situ clary sage cultivation over two successive years, combined or not with exogenous AMF inoculation, on the rhizospheric soil and root fungal communities. We obtained 1239 and 569 fungal amplicon sequence variants (ASV), respectively, in the rhizospheric soil and roots of under TE-polluted conditions. Remarkably, 69 AMF species were detected at our experimental site, belonging to 12 AMF genera. Furthermore, the inoculation treatment significantly shaped the fungal communities in soil and increased the number of AMF ASVs in clary sage roots. In addition, clary sage cultivation over successive years could be one of the explanatory parameters for the inter-annual variation in both fungal and AMF communities in the soil and root biotopes. Our data provide new insights on fungal and AMF communities in the rhizospheric soil and roots of an aromatic plant, clary sage, grown in TE-polluted agricultural soil.
土壤真菌群落在自然系统和农业生态系统中发挥着核心作用。因此，它们引起了广泛的研究兴趣。然而，诸如鼠尾草（Salvia sclarea L.）等芳香植物的真菌微生物群仍未得到充分研究。在微量元素（TE）污染的条件下以及植物修复方法的框架内尤其如此。土壤中高浓度的TE不仅会对微生物多样性和群落组成产生负面影响，还会影响植物的定植和生长。因此，本研究的目的是调查在前铅冶炼厂附近受TE污染的土壤中以及在鼠尾草种植条件下，土壤真菌和丛枝菌根真菌（AMF）的群落组成及其随时间的变化。我们使用Illumina MiSeq扩增子测序来评估连续两年原位种植鼠尾草（无论是否结合外源AMF接种）对根际土壤和根系真菌群落的影响。在TE污染条件下，我们分别在鼠尾草的根际土壤和根系中获得了1239个和569个真菌扩增子序列变体（ASV）。值得注意的是，在我们的实验地点检测到69种AMF物种，属于12个AMF属。此外，接种处理显著影响了土壤中的真菌群落，并增加了鼠尾草根中AMF ASV的数量。此外，连续多年种植鼠尾草可能是解释土壤和根际生物群落中真菌和AMF群落年际变化的参数之一。我们的数据为生长在TE污染农业土壤中的芳香植物鼠尾草根际土壤和根系中的真菌和AMF群落提供了新的见解。
