Johnson David, Vandenkoornhuyse Philippe J, Leake Jonathan R, Gilbert Lucy, Booth Rosemary E, Grime J Philip, Young J Peter W, Read David J
Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK.
These authors contributed equally to this work.
New Phytol. 2004 Feb;161(2):503-515. doi: 10.1046/j.1469-8137.2003.00938.x. Epub 2003 Dec 9.
• The diversity of arbuscular mycorrhizal (AM) fungi was investigated in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for 3 yr. • The experimental treatments comprised: bare soil; monocultures of the nonmycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP). The extent of AM colonization, shoot biomass and nitrogen and phosphorus concentrations were also measured. • The AM diversity was affected significantly by the floristic composition of the microcosms and shoot phosphorus concentration was positively correlated with AM diversity. The diversity of AM fungi in P. lanceolata decreased in the order: bare soil > C. flacca > 12 species > F. ovina. • The unexpectedly high diversity in the bare soil and sedge monoculture likely reflects differences in the modes of colonization and sources of inoculum in these treatments compared with the assemblages containing established AM-compatible plants.
• 在一片未施肥的石灰岩草原土壤中，对丛枝菌根（AM）真菌的多样性进行了调查，该土壤上生长着不同的合成维管植物组合，这些组合已经发育了3年。• 实验处理包括：裸土；非菌根营养型莎草羊胡子草的单种栽培；菌根营养型禾本科植物羊茅的单种栽培；以及由四种草本植物、四种禾本科植物和四种莎草组成的物种丰富的混合物。使用末端限制性片段长度多态性（T-RFLP）分析了披针叶车前生物测定幼苗根系中AM真菌的多样性。还测量了AM定殖程度、地上部生物量以及氮和磷浓度。• AM多样性受到微观世界植物区系组成的显著影响，地上部磷浓度与AM多样性呈正相关。披针叶车前中AM真菌的多样性按以下顺序降低：裸土＞羊胡子草＞12种植物组合＞羊茅。• 裸土和莎草单种栽培中出乎意料的高多样性可能反映了与含有已建立的AM兼容植物的组合相比，这些处理中定殖模式和接种源的差异。
