使用连续流动式细胞分离器的血液成分单采经验。

Apheresis experience with a continuous flow cell separator.

作者信息

Norol F, Aubert C, Scotto F, Duedari N

机构信息

Centre Départmental de Transfusion Sanguine du Val de Marne, Hôpital Henri Mondor, Creteil, France.

出版信息

Transfusion. 1988 Sep-Oct;28(5):470-3. doi: 10.1046/j.1537-2995.1988.28588337340.x.

DOI:10.1046/j.1537-2995.1988.28588337340.x

PMID:3420677

Abstract

The Dideco Vivacell separator is a continuous-flow centrifugation system that has only recently been used for cytapheresis. The authors' experience with this separator in 451 plateletpheresis and 164 leukapheresis procedures is presented. Platelet collection provided high platelet yields (9.53 +/- 2.85 X 10(11) with a collection efficiency of 74 +/- 14 percent for about 6 liters of total blood processed. Functional integrity was confirmed by normal in vitro tests (aggregation and response to hypotonic stress) and good in vivo recovery (55%). In leukapheresis, white cell yields were high (3.42 +/- 1.2 X 10(10) with 85 percent polymorphonuclear neutrophil cells. Their oxidative metabolism functions (generation of free oxygen radicals), investigated by chemiluminescence, were increased over donor values. Donor reactions, all of the mild citrate type, were rare.

摘要

迪德科维瓦塞尔血细胞分离机是一种连续流动离心系统，直到最近才用于细胞单采术。本文介绍了作者使用该分离机进行451次血小板单采和164次白细胞单采操作的经验。血小板采集获得了较高的血小板产量（9.53±2.85×10¹¹），在处理约6升全血时采集效率为74±14%。通过正常的体外试验（聚集和对低渗应激的反应）以及良好的体内回收率（55%）证实了功能完整性。在白细胞单采中，白细胞产量很高（3.42±1.2×10¹⁰），其中85%为多形核中性粒细胞。通过化学发光研究发现，它们的氧化代谢功能（游离氧自由基的产生）比供体值有所增加。供体反应很少见，均为轻度柠檬酸盐类型。