Dulnik Judyta, Sajkiewicz Paweł
Laboratory of Polymers and Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego 5b, 02-106 Warsaw, Poland.
Materials (Basel). 2021 Jun 18;14(12):3391. doi: 10.3390/ma14123391.
Four chemical crosslinking methods were used in order to prevent gelatin leaching in an aqueous environment, from bicomponent polycaprolactone/gelatin (PCL/Gt) nanofibers electrospun from an alternative solvent system. A range of different concentrations and reaction times were employed to compare genipin, 1-(3-dimethylaminopropyl)-N'-ethylcarbodimide hydrochloride/N-hydroxysuccinimide (EDC/NHS), 1,4-butanediol diglycidyl ether (BDDGE), and transglutaminase. The objective was to optimize and find the most effective method in terms of reaction time and solution concentration, that at the same time provides satisfactory gelatin crosslinking degree and ensures good morphology of the fibers, even after 24 h in aqueous medium in 37 °C. The series of experiments demonstrated that, out of the four compared crosslinking methods, EDC/NHS was able to yield satisfactory results with the lowest concentrations and the shortest reaction times.
为防止明胶在水性环境中从由替代溶剂体系静电纺丝得到的双组分聚己内酯/明胶（PCL/Gt）纳米纤维中浸出，采用了四种化学交联方法。使用了一系列不同的浓度和反应时间来比较京尼平、1-(3-二甲氨基丙基)-N'-乙基碳二亚胺盐酸盐/N-羟基琥珀酰亚胺（EDC/NHS）、1,4-丁二醇二缩水甘油醚（BDDGE）和转谷氨酰胺酶。目的是在反应时间和溶液浓度方面进行优化并找到最有效的方法，该方法同时能提供令人满意的明胶交联度，并确保即使在37℃的水性介质中放置24小时后纤维仍具有良好的形态。一系列实验表明，在四种比较的交联方法中，EDC/NHS能够在最低浓度和最短反应时间下产生令人满意的结果。
