Nik Mohamed Kamal Nik Nur Syazni, Abdul Aziz Fatin Athirah, Tan Wen-Nee, Fauzi Agustine Nengsih, Lim Vuanghao
Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam 13200, Kepala Batas, Penang, Malaysia.
Chemistry Section, School of Distance Education, Universsiti Sains Malaysia, Gelugor 11800, Penang, Malaysia.
Molecules. 2021 Jun 9;26(12):3518. doi: 10.3390/molecules26123518.
Pancreatic cancer is an aggressive disease that progresses in a relatively symptom-free manner; thus, is difficult to detect and treat. Essential oil is reported to exhibit pharmacological properties, besides its common and well-known function as aromatherapy. Therefore, this study herein aimed to investigate the anti-proliferative effect of essential oil extracted from leaves of (EO-L) against PANC-1 human pancreatic cancer cell line. The cell growth inhibitory concentration at 50% (IC) and selective index (SI) values of EO-L analyses were determined as 78 µg/mL and 1.23, respectively. Combination index (CI) analysis revealed moderate synergism (CI values of 0.36 to 0.75) between EO-L and 2 deoxy-d-glucose (2-DG) treatments. The treatments of PANC-1 cells with EO-L, 2-DG and EOL+2DG showed evidence of depolarization of mitochondrial membrane potential, cell growth arrest and apoptosis. The molecular mechanism causing the anti-proliferative effect between EO-L and 2-DG is potentially through pronounced up-regulation of (4.40-fold), (1.92-fold), (2.88-fold) and down-regulation of (0.11-fold), as supported by quantitative mRNA expression analysis. Collectively, the current data suggest that the combination of two anti-proliferative agents, EO-L and 2-DG, can potentially be explored as therapeutic treatments and as potentiating agents to conventional therapy against human pancreatic cancer.
胰腺癌是一种侵袭性疾病，其进展过程相对没有症状；因此，难以检测和治疗。据报道，精油除了具有作为芳香疗法的常见且广为人知的功能外，还具有药理特性。因此，本研究旨在调查从[植物名称]叶子中提取的精油（EO-L）对PANC-1人胰腺癌细胞系的抗增殖作用。EO-L分析的50%细胞生长抑制浓度（IC）和选择性指数（SI）值分别确定为78μg/mL和1.23。联合指数（CI）分析显示EO-L与2-脱氧-D-葡萄糖（2-DG）处理之间存在中度协同作用（CI值为0.36至0.75）。用EO-L、2-DG和EO-L+2-DG处理PANC-1细胞显示出线粒体膜电位去极化、细胞生长停滞和凋亡的证据。定量mRNA表达分析表明，EO-L和2-DG之间产生抗增殖作用的分子机制可能是通过[基因名称1]（4.40倍）、[基因名称2]（1.92倍）、[基因名称3]（2.88倍）的显著上调以及[基因名称4]（0.11倍）的下调。总体而言，目前的数据表明，两种抗增殖剂EO-L和2-DG的组合有可能作为治疗方法以及传统胰腺癌治疗的增效剂进行探索。
