Greenfield Edward A
Cold Spring Harb Protoc. 2021 Jul 1;2021(7):2021/7/pdb.prot103291. doi: 10.1101/pdb.prot103291.
Resistance to the most common antibiotics is what makes elimination of mycoplasma contamination so difficult, but not impossible. Different species of have varied sensitivities to each of the major classes of antibiotics. One method presented here entails selection in antibiotic-containing medium combined with single-cell cloning over activated macrophage feeders. Its success rate is as high as 70%. As a method of last resort, growing hybridoma cells as ascites tumors is one of the most effective methods of removing mycoplasma contamination. The mycoplasmas are removed from the hybridoma cell surface by the immune system of the mouse. Mice must be of the same genetic background as the hybridomas. This technique is the same as the method for ridding cells of bacterial or fungal infection, and the success rate is perhaps 80%.
对最常见抗生素产生耐药性是支原体污染难以消除但并非无法消除的原因。不同种类的支原体对各类主要抗生素的敏感性各不相同。这里介绍的一种方法是在含抗生素的培养基中进行筛选，并结合在活化巨噬细胞饲养层上进行单细胞克隆。其成功率高达70%。作为最后的手段，将杂交瘤细胞培养成腹水瘤是去除支原体污染最有效的方法之一。支原体通过小鼠的免疫系统从杂交瘤细胞表面清除。小鼠必须与杂交瘤具有相同的遗传背景。该技术与清除细胞细菌或真菌感染的方法相同，成功率可能为80%。
