Wang Dahao, Zhang Peng, Mei Xifan, Chen Zhenhua
Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
Jinzhou Medical University, Jinzhou 121001, China.
Regen Biomater. 2021 Jun 1;8(3):rbab022. doi: 10.1093/rb/rbab022. eCollection 2021 Jun.
In this article, we propose a simple scheme of using berberine (BBR) to modify porous calcium phosphate ceramics (named PCPC). These BBR molecules regulate the crystallization of hydroxyapatite nanorods on PCPC. We found that these nanorods and the adsorbed BBR changed the interface micro-environment of PCPC by SEM images. The microenvironment of PCPC surface is essential for promoting BMSCs' proliferation and differentiation. These results demonstrated that PCPC/BBR markedly improved the bone regeneration of osteoporosis rats. Moreover, PCPC/BBR had significantly increased the expression levels of ALP, osteocalcin and bone morphogenetic protein2 and RUNX2 in BMSCs originated from osteoporosis rats.
在本文中，我们提出了一种使用黄连素（BBR）修饰多孔磷酸钙陶瓷（命名为PCPC）的简单方案。这些BBR分子调节了PCPC上羟基磷灰石纳米棒的结晶。通过扫描电子显微镜图像，我们发现这些纳米棒和吸附的BBR改变了PCPC的界面微环境。PCPC表面的微环境对于促进骨髓间充质干细胞（BMSCs）的增殖和分化至关重要。这些结果表明，PCPC/BBR显著改善了骨质疏松大鼠的骨再生。此外，PCPC/BBR显著提高了源自骨质疏松大鼠的BMSCs中碱性磷酸酶（ALP）、骨钙素、骨形态发生蛋白2和RUNX2的表达水平。
