CREA Centro di Ricerca Difesa e Certificazione, Via C.G. Bertero 22, 00156, Roma, Italy.
Dipartimento di AGRARIA, Università degli Studi Mediterranea di Reggio Calabria, Località Feo di Vito, 89122, Reggio Calabria, Italy.
Arch Virol. 2021 Sep;166(9):2619-2621. doi: 10.1007/s00705-021-05146-3. Epub 2021 Jul 2.
The genome of a new carlavirus isolate from asymptomatic wild Capparis spinosa L. plants in Sicily was sequenced via high-throughput sequencing (HTS) and 5'/3' RACE experiments. The complete genomic sequence was found to be 8,280 nt in length, excluding the poly(A) tail, and contained five putative open reading frames (ORFs). Molecular characterization revealed a close relationship to caper latent virus (CapLV), with 87% and 90% nucleotide sequence identity to available partial sequences of the ORFs encoding the replicase and coat protein of that virus. According to the molecular criteria for species demarcation, which is based on the ORF-1- and ORF-5-encoded proteins, the virus characterized in this study could be considered a variant of CapLV, and we have thus designated it as CapLV-W.
通过高通量测序（HTS）和 5'/3' RACE 实验，对来自西西里岛无症状野生芹菜植株中的一种新的卡拉病毒分离株的基因组进行了测序。发现该完整基因组序列不含 poly(A) 尾，长度为 8280nt，包含五个推定的开放阅读框（ORF）。分子特征分析显示，与芹菜潜隐病毒（CapLV）密切相关，与该病毒的复制酶和外壳蛋白编码 ORF 的可用部分序列的核苷酸序列同一性分别为 87%和 90%。根据基于 ORF-1 和 ORF-5 编码蛋白的种间划分的分子标准，本研究中鉴定的病毒可被视为 CapLV 的一个变体，因此我们将其命名为 CapLV-W。
