Department of Interventional Radiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450001, China.
Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450001, China.
Colloids Surf B Biointerfaces. 2021 Oct;206:111949. doi: 10.1016/j.colsurfb.2021.111949. Epub 2021 Jun 29.
Antibacterial and antihyperplasia airway stents are highly desirable for tracheal stenosis. Herein, a series of polylactic acid (PLA) and silver nanoparticles (AgNPs) nanofiber membranes (PLA, PLA-4 %AgNPs and PLA-6 % AgNPs) were prepared by electrospinning. The physicochemical and biological properties of the resultant nanofiber membranes were examined. The SEM and drug release results indicated that the AgNPs were successfully introduced into PLA, and could be sustained to be released from membranes. The membranes showed antibacterial activity against S. aureus and P. aeruginosa, and cytocompatibility towards CCC-HPF-1 and NHBE cells. Furthermore, the membranes were used to cover a self-expandable metallic stent for use in the treatment of rabbit tracheal stenosis. The in vivo results revealed that the membranes, especially the AgNPs-coated airway stent could suppress tracheal stenosis by reducing inflammation and collagen deposition. Additionally, the study further confirmed that the inhibition of bacterial content in the trachea could be positively correlated with the reduction in tracheal granulation tissue hyperplasia. Conclusively, the PLA/AgNPs nanofiber membrane-coated airway stent has practical value for patients with clinical tracheal stenosis.
抗菌和抗气道增生支架对于气管狭窄是非常理想的。在此，我们通过静电纺丝制备了一系列聚乳酸（PLA）和载银纳米粒子（AgNPs）纳米纤维膜（PLA、PLA-4%AgNPs 和 PLA-6%AgNPs）。研究了所得纳米纤维膜的物理化学和生物学性能。SEM 和药物释放结果表明，AgNPs 已成功引入 PLA 中，并且可以从膜中持续释放。该膜对金黄色葡萄球菌和铜绿假单胞菌具有抗菌活性，对 CCC-HPF-1 和 NHBE 细胞具有细胞相容性。此外，该膜被用于覆盖自膨式金属支架以治疗兔气管狭窄。体内结果表明，该膜，特别是载银纳米粒子的气道支架，通过减少炎症和胶原沉积来抑制气管狭窄。此外，研究进一步证实，气管中细菌含量的抑制与气管肉芽组织增生的减少呈正相关。总之，PLA/AgNPs 纳米纤维膜涂层气道支架对临床气管狭窄患者具有实用价值。
