Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Life Sci. 2021 Oct 1;282:119602. doi: 10.1016/j.lfs.2021.119602. Epub 2021 Jul 2.
The application of electroactive scaffolds can be promising for bone tissue engineering applications. In the current paper, we aimed to fabricate an electro-conductive scaffold based on carbon nanofibers (CNFs) containing ferrous sulfate. FeSO·7HO salt with different concentrations 5, 10, and 15 wt%, were blended with polyacrylonitrile (PAN) polymer as the precursor and converted to FeO3/CNFs nanocomposite by electrospinning and heat treatment. The characterization was conducted using SEM, EDX, XRD, FTIR, and Raman methods. The results showed that the incorporation of Fe salt induces no adverse effect on the nanofibers' morphology. EDX analysis confirmed that the Fe ions are uniformly dispersed throughout the CNF mat. FTIR spectroscopy showed the interaction of Fe salt with PAN polymer. Raman spectroscopy showed that the incorporation of FeSO4·7HO reduced the ID/IG ratio, indicating more ordered carbon in the synthesized nanocomposite. Electrical resistance measurement depicted that, although the incorporation of ferrous sulfate reduced the electrical conductivity, the conductive is suitable for electrical stimulation. The in vitro studies revealed that the prepared nanocomposites were cytocompatible and only negligible toxicity (less than 10%) induced by CNFs/FeO fabricated from PAN FeSO·7HO 15%. Although various nanofibrous composite fabricated with Fe NPs have been evaluated for tissue engineering applications, CNFs exhibited promising properties, such as excellent mechanical strength, biocompatibility, and electrical conductivity. These results showed that the fabricated nanocomposites could be applied as the bone tissue engineering scaffold.
基于含有硫酸亚铁的碳纤维（CNF）制备了一种导电支架。将不同浓度 5、10 和 15 wt% 的 FeSO·7HO 盐与聚丙烯腈（PAN）聚合物混合作为前体，并通过静电纺丝和热处理转化为 FeO3/CNF 纳米复合材料。使用 SEM、EDX、XRD、FTIR 和 Raman 方法进行了表征。结果表明，Fe 盐的掺入对纳米纤维的形态没有不利影响。EDX 分析证实，Fe 离子均匀分散在 CNF 垫中。傅里叶变换红外光谱显示 Fe 盐与 PAN 聚合物相互作用。拉曼光谱表明，FeSO4·7HO 的掺入降低了 ID/IG 比，表明合成纳米复合材料中存在更多有序的碳。电阻测量表明，尽管掺入硫酸亚铁降低了电导率，但导电性适合电刺激。体外研究表明，所制备的纳米复合材料具有细胞相容性，由 PAN FeSO·7HO 15% 制备的 CNFs/FeO 仅引起轻微毒性（小于 10%）。尽管已经评估了各种含有 Fe NPs 的纳米纤维复合材料用于组织工程应用，但 CNFs 表现出优异的机械强度、生物相容性和导电性等有前景的特性。这些结果表明，所制备的纳米复合材料可用作骨组织工程支架。
