Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Department of Microsystems Engineering, Rochester Institute of Technology, Rochester, NY, USA; Department of Biomedical Engineering, Rochester Institute of Technology, Rochester, NY, USA.
Mater Sci Eng C Mater Biol Appl. 2020 Dec;117:111226. doi: 10.1016/j.msec.2020.111226. Epub 2020 Jul 3.
Bone tissue engineering is a new and applicable emerging approach to repair bone defects. Electrical conductive scaffolds through a physiologically relevant physical signaling, i.e., electrical stimulation, are highly promising candidates for tissue engineering applications. In this paper, we fabricated carbon nanofiber/gold nanoparticle (CNF/AuNP) conductive scaffolds using two distinct methods. These methods are blending electrospinning in which AuNPs were blended with electrospinning solution, and electrospinning/electrospraying in which AuNPs were electrosprayed simultaneously with electrospinning. The obtained electrospun mats underwent a stabilization/carbonization process. The scaffolds were characterized by SEM, XRD, FT-IR, and Raman spectroscopy. SEM characterizations showed improved morphology and a slight decrease in the diameter of the electrospinned and electrosprayed nanofibers (from 178.66 ± 38.40 nm to 157.94 ± 24.14 nm and 120.81 ± 13.77 nm, respectively). Raman spectroscopy showed improvement in the graphitization. Electrical conductivity improved by up to 29.2% and 81% in electrospraying and blending electrospinning modes, respectively. Indirect MTT and LDH toxicity assays directly were performed to assess MG63 cell toxicity, but no significant toxicity was observed, and the scaffolds did not adversely affect cell proliferation. It can be concluded these scaffolds have the potential for bone tissue engineering applications.
骨组织工程是一种新的、适用的新兴方法，可用于修复骨缺损。通过生理相关的物理信号（即电刺激）的导电支架是组织工程应用的有前途的候选者。在本文中，我们使用两种不同的方法制备了碳纳米纤维/金纳米粒子（CNF/AuNP）导电支架。这两种方法是混合静电纺丝，其中 AuNPs 与静电纺丝溶液混合，以及静电纺丝/静电喷涂，其中 AuNPs 与静电纺丝同时静电喷涂。所得的静电纺丝毡经历了稳定/碳化过程。通过 SEM、XRD、FT-IR 和拉曼光谱对支架进行了表征。SEM 表征显示出改进的形态和电纺和电喷纳米纤维直径的轻微降低（从 178.66 ± 38.40nm 分别降至 157.94 ± 24.14nm 和 120.81 ± 13.77nm）。拉曼光谱显示出石墨化程度的提高。在静电喷涂和混合静电纺丝模式下，电导率分别提高了 29.2%和 81%。通过间接 MTT 和 LDH 毒性测定直接评估 MG63 细胞毒性，但未观察到明显毒性，并且支架未对细胞增殖产生不利影响。可以得出结论，这些支架具有用于骨组织工程应用的潜力。
