Ozkan Kubra, Bekiroglu Hatice, Cebi Nur, Bozkurt Fatih, Dere Sevda, Ozdemir Hilmi, Arici Muhammet, Karasu Salih, Sagdic Osman
Department of Nutrition and Dietetics, Health Sciences Faculty, Istinye University, 34010 Istanbul, Türkiye.
Department of Food Engineering, Faculty of Agriculture, Sirnak University, 73300 Sirnak, Türkiye.
Foods. 2025 Jul 22;14(15):2562. doi: 10.3390/foods14152562.
This study employed response surface methodology for the first-time optimization of the ultrasound-assisted extraction (UAE) of the total phenolic content (TPC) and ABTS from edible lichens, including , , , , and . Fourteen experimental points were generated using Design Expert Software, with the extraction temperature (25-40 °C), extraction time (5-20 min), and ethanol concentration (0-80%) as independent variables, and TPC and ABTS as dependent variables. The phenolic profile and mineral and antibacterial properties of the optimized lichen extracts were determined. Evernic and usnic acid were found in species. Atranorin was detected only in . Fumarprotocetraric acid was found exclusively in and was not detected in any of the other lichens. Calcium was found to have the highest mineral content in all the lichens, followed by potassium. , showing the lowest inhibition effect against all tested bacteria, while exhibited the most effective inhibition.
本研究首次采用响应面法对包括[具体种类1]、[具体种类2]、[具体种类3]、[具体种类4]和[具体种类5]在内的可食用地衣中总酚含量（TPC）和ABTS的超声辅助提取（UAE）进行优化。使用Design Expert软件生成了14个实验点，以提取温度（25 - 40°C）、提取时间（5 - 20分钟）和乙醇浓度（0 - 80%）作为自变量，TPC和ABTS作为因变量。测定了优化后的地衣提取物的酚类概况、矿物质和抗菌性能。在[具体种类1]中发现了扁枝衣酸和松萝酸。仅在[具体种类2]中检测到黑茶渍素。富马原岛衣酸仅在[具体种类3]中发现，在其他任何地衣中均未检测到。发现所有地衣中钙的矿物质含量最高，其次是钾。[具体种类4]对所有测试细菌的抑制作用最低，而[具体种类5]表现出最有效的抑制作用。
