链脲佐菌素诱导的糖尿病中棕色脂肪组织线粒体过程的变化
Changes in brown-adipose-tissue mitochondrial processes in streptozotocin-diabetes.
作者信息
Jamal Z, Saggerson E D
机构信息
Department of Biochemistry, University College London, U.K.
出版信息
Biochem J. 1988 May 15;252(1):293-6. doi: 10.1042/bj2520293.
Abstract
Diabetic rats were used as a source of brown-adipose-tissue mitochondria 2 days after a single subcutaneous injection of streptozotocin (100 mg/kg). Diabetes caused an 80% decrease in carnitine-dependent oxidation of palmitoyl-CoA and a 50-60% decrease in overt carnitine palmitoyltransferase activity. An additional lesion in brown-adipose-tissue mitochondrial oxidative capacity was also indicated, since diabetes increased by 30-50% the rate of oxidation under uncoupled conditions of several respiratory substrates (i.e. malate + palmitoylcarnitine, malate + pyruvate, succinate, NNN'N'-tetramethyl-p-phenylenediamine + ascorbate). This decrease in mitochondrial function was accompanied by an approx. 30% decrease in the abundance of cytochromes (a + a3) and total cytochromes b.
摘要
单次皮下注射链脲佐菌素（100 mg/kg）2天后，将糖尿病大鼠作为棕色脂肪组织线粒体的来源。糖尿病导致棕榈酰辅酶A的肉碱依赖性氧化减少80%，明显的肉碱棕榈酰转移酶活性降低50 - 60%。还表明棕色脂肪组织线粒体氧化能力存在额外损伤，因为糖尿病使几种呼吸底物在解偶联条件下（即苹果酸+棕榈酰肉碱、苹果酸+丙酮酸、琥珀酸、N，N，N'，N'-四甲基对苯二胺+抗坏血酸）的氧化速率提高了30 - 50%。线粒体功能的这种下降伴随着细胞色素（a + a3）和总细胞色素b丰度约30%的降低。
相似文献
1
Changes in brown-adipose-tissue mitochondrial processes in streptozotocin-diabetes.链脲佐菌素诱导的糖尿病中棕色脂肪组织线粒体过程的变化
Biochem J. 1988 May 15;252(1):293-6. doi: 10.1042/bj2520293.
2
Differentiation of rapid and slower-acting effects of insulin on mitochondrial processes in brown adipose tissue from streptozotocin-diabetic rats.链脲佐菌素诱导的糖尿病大鼠棕色脂肪组织中线粒体过程中胰岛素快速和缓慢作用的差异。
Biochem J. 1989 Feb 15;258(1):309-11. doi: 10.1042/bj2580309.
3
Regulation of respiratory activity of brown adipose tissue mitochondria through changes in cytochromes.通过细胞色素变化对棕色脂肪组织线粒体呼吸活性的调节
Biotechnol Appl Biochem. 1990 Oct;12(5):562-6.
4
Some aspects of fatty acid oxidation in isolated fat-cell mitochondria from rat.大鼠分离脂肪细胞线粒体中脂肪酸氧化的某些方面。
Biochem J. 1975 Dec;152(3):485-94. doi: 10.1042/bj1520485.
5
Hepatic mitochondrial inner membrane properties and carnitine palmitoyltransferase A and B. Effect of diabetes and starvation.肝线粒体内膜特性以及肉碱棕榈酰转移酶A和B。糖尿病和饥饿的影响。
Biochem J. 1985 Dec 1;232(2):445-50. doi: 10.1042/bj2320445.
6
Sensitivity of brown-adipose-tissue carnitine palmitoyltransferase to inhibition by malonyl-CoA.棕色脂肪组织肉碱棕榈酰转移酶对丙二酰辅酶A抑制作用的敏感性。
Biochem J. 1982 Apr 15;204(1):373-5. doi: 10.1042/bj2040373.
7
[Mitochondria from brown and white adipose tissue: structure, enzyme profile and oxidative phosphorylation].[棕色和白色脂肪组织中的线粒体：结构、酶谱与氧化磷酸化]
Hoppe Seylers Z Physiol Chem. 1970 Dec;351(12):1513-23.
8
Role of carnitine palmitoyltransferase I in the regulation of hepatic ketogenesis during the onset and reversal of chronic diabetes.肉碱棕榈酰转移酶I在慢性糖尿病发生及逆转过程中对肝脏生酮作用调节中的作用
Biochem J. 1988 Jan 15;249(2):409-14. doi: 10.1042/bj2490409.
9
Hepatic mitochondrial function in ketogenic states. Diabetes, starvation, and after growth hormone administration.生酮状态下的肝脏线粒体功能。糖尿病、饥饿以及生长激素给药后。
J Clin Invest. 1975 Jun;55(6):1237-44. doi: 10.1172/JCI108042.
10
The H+/e- stoicheiometry of respiration-linked proton translocation in the cytochrome system of mitochondria.线粒体细胞色素系统中呼吸链质子转运的H⁺/e⁻化学计量关系。
Biochem J. 1980 Oct 15;192(1):203-18. doi: 10.1042/bj1920203.
引用本文的文献
1
Deficiency Attenuates Diet-Induced Obesity and Insulin Resistance by Promoting Fatty Acid Oxidation and Thermogenesis in Brown Adipocytes.缺乏通过促进棕色脂肪细胞中的脂肪酸氧化和产热来减轻饮食诱导的肥胖和胰岛素抵抗。
Adv Sci (Weinh). 2021 Feb 1;8(6):2002794. doi: 10.1002/advs.202002794. eCollection 2021 Mar.
2
Carnitine Palmitoyltransferase 1 Increases Lipolysis, UCP1 Protein Expression and Mitochondrial Activity in Brown Adipocytes.肉碱棕榈酰转移酶1增强棕色脂肪细胞中的脂肪分解、解偶联蛋白1（UCP1）的蛋白表达及线粒体活性。
PLoS One. 2016 Jul 20;11(7):e0159399. doi: 10.1371/journal.pone.0159399. eCollection 2016.
3
Fatty acid metabolism and the basis of brown adipose tissue function.脂肪酸代谢与棕色脂肪组织功能的基础。
Adipocyte. 2015 Dec 10;5(2):98-118. doi: 10.1080/21623945.2015.1122857. eCollection 2016 Apr-Jun.
4
Curcumin analogues as selective fluorescence imaging probes for brown adipose tissue and monitoring browning.姜黄素类似物作为棕色脂肪组织的选择性荧光成像探针及监测棕色化过程。
Sci Rep. 2015 Aug 13;5:13116. doi: 10.1038/srep13116.
5
Increased mitochondrial activity in BMP7-treated brown adipocytes, due to increased CPT1- and CD36-mediated fatty acid uptake.BMP7 处理后的棕色脂肪细胞中线粒体活性增加，这是由于 CPT1 和 CD36 介导的脂肪酸摄取增加所致。
Antioxid Redox Signal. 2013 Jul 20;19(3):243-57. doi: 10.1089/ars.2012.4536. Epub 2012 Oct 9.
6
Differentiation of rapid and slower-acting effects of insulin on mitochondrial processes in brown adipose tissue from streptozotocin-diabetic rats.链脲佐菌素诱导的糖尿病大鼠棕色脂肪组织中线粒体过程中胰岛素快速和缓慢作用的差异。
Biochem J. 1989 Feb 15;258(1):309-11. doi: 10.1042/bj2580309.
7
Effects of hypothyroidism and hyperthyroidism on GDP binding to brown-adipocyte mitochondria from rats.甲状腺功能减退和甲状腺功能亢进对大鼠棕色脂肪细胞线粒体中GDP结合的影响。
Biochem J. 1989 Oct 15;263(2):341-5. doi: 10.1042/bj2630341.
8
Activities of enzymes of glycerolipid synthesis in brown adipose tissue after treatment of rats with the adrenergic agonists BRL 26830A and phenylephrine, after exposure to cold and in streptozotocin-diabetes.用肾上腺素能激动剂BRL 26830A和去氧肾上腺素处理大鼠后、暴露于寒冷环境后以及在链脲佐菌素诱导的糖尿病状态下，棕色脂肪组织中甘油olipid合成酶的活性。 （注：原文中“glycerolipid”可能有误，推测应为“glycerolipid”，即甘油脂质 ）
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):665-9. doi: 10.1042/bj2770665.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Carnitine acyltransferase activities in rat liver and heart measured with palmitoyl-CoA and octanoyl-CoA. Latency, effects of K+, bivalent metal ions and malonyl-CoA.用棕榈酰辅酶A和辛酰辅酶A测定大鼠肝脏和心脏中的肉碱酰基转移酶活性。潜伏性、钾离子、二价金属离子和丙二酰辅酶A的影响。
Biochem J. 1982 Feb 15;202(2):397-405. doi: 10.1042/bj2020397.
3
Malonyl CoA inhibition of carnitine acyltransferase activities: effects of thiol-group reagents.丙二酰辅酶A对肉碱酰基转移酶活性的抑制作用：硫醇基团试剂的影响
FEBS Lett. 1982 Jan 11;137(1):124-8. doi: 10.1016/0014-5793(82)80329-3.
4
A role for insulin in the diet-induced thermogenesis of cafeteria-fed rats.胰岛素在自由选择饮食喂养的大鼠饮食诱导产热中的作用。
Metabolism. 1981 Jul;30(7):673-8. doi: 10.1016/0026-0495(81)90082-2.
5
Glucagon treatment of rats inhibits the accumulation of lysophospholipids by liver mitochondria during preparation and subsequent incubation.用胰高血糖素处理大鼠，可抑制肝线粒体在制备过程及随后的温育过程中溶血磷脂的积累。
Biosci Rep. 1984 Nov;4(11):903-8. doi: 10.1007/BF01116887.
6
Altered sensitivity of carnitine palmitoyltransferase to inhibition by malonyl-CoA in ketotic diabetic rats.酮症糖尿病大鼠中肉碱棕榈酰转移酶对丙二酰辅酶A抑制作用的敏感性改变。
Biochem J. 1984 Apr 1;219(1):337-9. doi: 10.1042/bj2190337.
7
Modulation of beta-oxidation and proton conductance pathway of brown adipose tissue in hypo- and hyperinsulinemic states.低胰岛素血症和高胰岛素血症状态下棕色脂肪组织β-氧化和质子传导途径的调节
FEBS Lett. 1984 Jan 23;166(1):141-5. doi: 10.1016/0014-5793(84)80060-5.
8
Role of insulin in thermogenic responses to refeeding in 3-day-fasted rats.胰岛素在禁食3天的大鼠再喂养热生成反应中的作用。
Am J Physiol. 1983 Aug;245(2):E160-5. doi: 10.1152/ajpendo.1983.245.2.E160.
9
Brown adipose tissue metabolism in streptozotocin-diabetic rats.链脲佐菌素诱导的糖尿病大鼠棕色脂肪组织代谢
Endocrinology. 1983 Aug;113(2):604-10. doi: 10.1210/endo-113-2-604.
10
A modified fluorometric micromethod for DNA.一种改良的DNA荧光微量法。
Clin Chim Acta. 1971 Apr;32(2):203-6. doi: 10.1016/0009-8981(71)90333-0.
Zotero 插件