Júnior Abimael E Silva, Vasconcelos Priscylla C, Saraiva Mauro M S, Filho Lauro Santos, Silva Núbia M V, Givisiez Patricia E N, Oliveira Celso J B
Department of Animal Science, College for Agricultural Sciences, Federal University of Paraiba, Brazil.
Department of Pharmaceutical Sciences, College of Health Sciences, Federal University of Paraiba, Brazil.
Vet World. 2021 May;14(5):1074-1079. doi: 10.14202/vetworld.2021.1074-1079. Epub 2021 May 5.
Antimicrobial resistance poses a major threat to global public health. Foodstuff of animal origin can serve as potential vehicles for the dissemination of antimicrobial-resistant bacteria and resistance genes to consumers. In view of the lack of knowledge about antimicrobial resistance in bacteria associated with goat milk, the aim of this study was to report species-level identification and antimicrobial susceptibility profiles of a large collection of spp. isolates recovered from raw goat milk in Brazil.
A total of 434 spp. isolates originated from 510 goat milk samples in Northeast Brazil were investigated. The isolates were obtained by conventional microbiological methods. Species identification and antimicrobial susceptibility testing were performed by means of a semi-automated system using a panel for biochemical tests and broth microdilution method for 19 antimicrobial drugs.
Although (22.6%) accounted for the majority of the isolates, a total of 13 different non- staphylococci spp. were identified. High resistance rates against erythromycin (40.8%), and the beta-lactams ampicillin (45.9%) and penicillin (42.9%) were observed among isolates. The most significant findings were related to the resistance against quinupristin-dalfopristin, a drug of last resort used in human medicine to treat infections caused by vancomycin-resistant and enterococci.
The high diversity of spp. showing phenotypic resistance against different antimicrobial drugs encourages further investigations on the real impact of these bacteria as reservoirs of antimicrobial resistance genes to consumers. Furthermore, the potential impact of technological processes, such as pasteurization, fermentation, and maturation, on the maintenance and dissemination of antimicrobial resistance among the microbial populations in milk and dairy products must also be investigated.
抗菌药物耐药性对全球公共卫生构成重大威胁。动物源性食品可能成为向消费者传播抗菌药物耐药菌及耐药基因的潜在载体。鉴于对与山羊奶相关细菌的抗菌药物耐药性了解不足，本研究旨在报告从巴西生山羊奶中分离出的大量 spp. 菌株的种水平鉴定及抗菌药物敏感性概况。
对来自巴西东北部510份山羊奶样品的434株 spp. 菌株进行了研究。通过传统微生物学方法获得这些菌株。采用半自动系统进行种属鉴定及抗菌药物敏感性测试，该系统使用生化测试板及针对19种抗菌药物的肉汤微量稀释法。
尽管 （22.6%）占分离菌株的大多数，但共鉴定出13种不同的非 葡萄球菌属 spp.。在 菌株中观察到对红霉素（40.8%）、β-内酰胺类氨苄西林（45.9%）和青霉素（42.9%）的高耐药率。最显著的发现与对奎奴普丁-达福普汀的耐药性有关，该药物是用于治疗由耐万古霉素 及肠球菌引起感染的人类医学最后手段药物。
显示出对不同抗菌药物表型耐药的 spp. 的高度多样性，促使进一步研究这些细菌作为抗菌药物耐药基因储库对消费者的实际影响。此外，还必须研究诸如巴氏杀菌、发酵和成熟等工艺过程对牛奶及乳制品中微生物群体抗菌药物耐药性的维持和传播的潜在影响。
