Gołaś-Prądzyńska Marlena, Rola Jolanta G
Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Puławy, Poland.
J Vet Res. 2021 Dec 20;65(4):449-455. doi: 10.2478/jvetres-2021-0071. eCollection 2021 Dec.
Enterococci are widespread, being part of the bacterial flora of humans and animals. The food chain can be therefore considered as the main route of transmission of antibiotic resistant bacteria between the animal and human populations. Milk in particular represents a source from which resistant bacteria can enter the human food chain. The aim of the study was to determine the occurrence and resistance to antimicrobial agents of spp. strains isolated from raw goat's milk samples.
A total of 207 goat's milk samples were collected. Samples were cultivated on selective media and confirmed as or and screened for selected resistance genes by PCR. Drug susceptibility determination was performed by microdilution on Sensititre EU Surveillance Enterococcus EUVENC Antimicrobial Susceptibility Testing (AST) Plates and Sensititre US National Antimicrobial Resistance Monitoring System Gram Positive CMV3AGPF AST Plates.
Enterococcal strains totalling 196 were isolated, of which 40.8% were and 15.3% were . All tested isolates were susceptible to linezolid, penicillin and tigecycline. For most other antimicrobials the prevalence of resistance was 0.5-6.6% while high prevalence of quinupristin/dalfopristin (51.5%), tetracycline (30%) and lincomycin (52%) resistance was observed.
This study affords better knowledge concerning the safety of raw goat's milk in terms of the enterococci possible to isolate from this foodstuff. It seems that enterococci in milk are still mostly susceptible to antimicrobials of major concern as multiply resisted drugs, such as gentamycin and vancomycin. However, the presence of multi-resistant strains in goat milk is cause for apprehension.
肠球菌分布广泛，是人和动物细菌菌群的一部分。因此，食物链可被视为动物和人类群体之间抗生素耐药菌的主要传播途径。特别是牛奶，它是耐药菌进入人类食物链的一个来源。本研究的目的是确定从生山羊奶样品中分离出的 菌属菌株的发生率及其对抗菌剂的耐药性。
共收集了 207 份山羊奶样品。将样品在选择性培养基上培养，确认为 或 ，并通过 PCR 筛选选定的耐药基因。通过在 Sensititre EU 监测肠球菌 EUVENC 抗菌药敏试验（AST）平板和 Sensititre 美国国家抗菌药物耐药性监测系统革兰氏阳性 CMV3AGPF AST 平板上进行微量稀释来测定药物敏感性。
共分离出 196 株肠球菌菌株，其中 40.8% 为 ，15.3% 为 。所有测试菌株对利奈唑胺、青霉素和替加环素敏感。对于大多数其他抗菌药物，耐药率为 0.5 - 6.6%，而观察到对奎奴普丁/达福普汀（51.5%）、四环素（30%）和林可霉素（52%）的高耐药率。
本研究在从这种食品中可能分离出的肠球菌方面，提供了关于生山羊奶安全性的更好认识。似乎牛奶中的肠球菌对作为多重耐药药物的主要关注抗菌药物，如庆大霉素和万古霉素，大多仍敏感。然而，山羊奶中存在多重耐药菌株令人担忧。
